公开(公告)号：US20220025422A1

公开(公告)日：2022-01-27

申请号：US17296932

申请日：2019-11-26

Applicant: Novozymes A/S

Inventor： Suchindra Maiyuran ,  Sarah McFarland

IPC: C12P21/02 ,  C12N15/80 ,  C07K14/37

Abstract: The present invention relates to an isolated variant Ire1 polypeptide, comprising (a) an amino acid substitution at position 153 of the Trichoderma reesei Ire1 polypeptide of SEQ ID NO: 2; or (b) an amino acid substitution at a position corresponding to position 153 of the Trichoderma reesei Ire1 of SEQ ID NO: 2 and has at least 70%, but less than 100%, sequence identity to the amino acid sequence of SEQ ID NO: 2. The present invention also relates to a recombinant filamentous fungal host cell comprising the mutated ire1 gene encoding the variant Ire1 polypeptide for producing a secreted heterologous polypeptide of interest, a method for producing the secreted heterologous polypeptide of interest in the recombinant filamentous fungal host cell, and a method of improving the productivity or yield of the secreted heterologous polypeptide of interest in the recombinant filamentous fungal host cell.