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    Methods For Eliminating Or Reducing The Expression Of A Gene In A Filamentous Fungal Strain
    2.
    发明申请
    Methods For Eliminating Or Reducing The Expression Of A Gene In A Filamentous Fungal Strain 审中-公开
    消除或减少丝状真菌菌株中基因表达的方法

    公开(公告)号：US20140287475A1

    公开(公告)日：2014-09-25

    申请号：US14255642

    申请日：2014-04-17

    Applicant: Novozymes A/S ,  Novozymes, Inc.

    Inventor： Howard Brody ,  Suchindra Maiyuran ,  Hiroaki Udagawa

    IPC: C12P1/02

    CPC classification number: C12P1/02 ,  C12N15/113 ,  C12N15/1137 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53

    Abstract: The present invention relates to methods for reducing or eliminating the expression of a target gene in a filamentous fungal strain, comprising: (a) inserting into the genome of the filamentous fungal strain a double-stranded transcribable nucleic acid construct comprising a first nucleotide sequence comprising a promoter operably linked to a homologous coding region of the target gene and a second nucleotide sequence comprising the homologous coding region, or a portion thereof, of the target gene, wherein the first and second nucleotide sequences are complementary to each other and the second nucleotide sequence is in reverse orientation relative to the first nucleotide sequence; and (b) inducing production of an interfering RNA encoded by the double-stranded transcribable nucleic acid construct by cultivating the filamentous fungal strain under conditions conducive for production of the interfering RNA; wherein the interfering RNA interacts with RNA transcripts of the target gene to reduce or eliminate expression of the target gene. The present invention also relates to the filamentous fungal strains and to methods of producing a biological substance of interest in such filamentous fungal strains.

    Abstract translation: 本发明涉及用于减少或消除丝状真菌菌株中靶基因表达的方法，其包括：（a）将丝状真菌菌株的双链可转录核酸构建体插入基因组中，所述双链可转录核酸构建体包含第一核苷酸序列，其包含 可操作地连接到靶基因的同源编码区的启动子和包含靶基因的同源编码区或其部分的第二核苷酸序列，其中第一和第二核苷酸序列彼此互补，第二核苷酸 序列相对于第一个核苷酸序列处于相反方向; 和（b）通过在有利于产生干扰RNA的条件下培养丝状真菌菌株诱导由双链可转录核酸构建体编码的干扰RNA的产生; 其中所述干扰RNA与所述靶基因的RNA转录物相互作用以减少或消除所述靶基因的表达。 本发明还涉及丝状真菌菌株以及在这种丝状真菌菌株中生产目的生物物质的方法。

    Methods For Increasing The Productivity Of A Filamentous Fungal Cell In The Production Of A Polypeptide
    3.
    发明申请
    Methods For Increasing The Productivity Of A Filamentous Fungal Cell In The Production Of A Polypeptide 有权

    公开(公告)号：US20220064228A1

    公开(公告)日：2022-03-03

    申请号：US17413434

    申请日：2019-12-12

    Applicant: Novozymes A/S

    Inventor： Suchindra Maiyuran ,  Alfredo Lopez De Leon

    IPC: C07K14/37 ,  C12N9/24 ,  C12P21/00

    Abstract: The present invention relates to a mutant of a parent filamentous fungal cell, comprising a coding sequence of a polypeptide of interest under the transcriptional control of a promoter regulated by a transcription factor, wherein the gene encoding the transcription factor is modified in the parent filamentous fungal cell to produce the mutant rendering the mutant deficient in the production of the transcription factor, which increases the productivity of the mutant in the production of the polypeptide of interest, and/or reduces or eliminates the cellulase-negative phenotype in the resulting mutant. The present invention also relates to a method for constructing such a mutant and a method of producing a polypeptide of interest with such a mutant.

    Improved Filamentous Fungal Host Cells
    5.
    发明申请
    Improved Filamentous Fungal Host Cells 有权

    公开(公告)号：US20220025422A1

    公开(公告)日：2022-01-27

    申请号：US17296932

    申请日：2019-11-26

    Applicant: Novozymes A/S

    Inventor： Suchindra Maiyuran ,  Sarah McFarland

    IPC: C12P21/02 ,  C12N15/80 ,  C07K14/37

    Abstract: The present invention relates to an isolated variant Ire1 polypeptide, comprising (a) an amino acid substitution at position 153 of the Trichoderma reesei Ire1 polypeptide of SEQ ID NO: 2; or (b) an amino acid substitution at a position corresponding to position 153 of the Trichoderma reesei Ire1 of SEQ ID NO: 2 and has at least 70%, but less than 100%, sequence identity to the amino acid sequence of SEQ ID NO: 2. The present invention also relates to a recombinant filamentous fungal host cell comprising the mutated ire1 gene encoding the variant Ire1 polypeptide for producing a secreted heterologous polypeptide of interest, a method for producing the secreted heterologous polypeptide of interest in the recombinant filamentous fungal host cell, and a method of improving the productivity or yield of the secreted heterologous polypeptide of interest in the recombinant filamentous fungal host cell.

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