公开(公告)号：US20250043227A1

公开(公告)日：2025-02-06

申请号：US18362312

申请日：2023-07-31

Applicant: ORIENTAL SYSTEM TECHNOLOGY INC.

Inventor： Chein-Hsun WANG ,  Yun-Hsiang CHEN ,  Jyh-Yih LEU ,  Wen-Chie HUANG ,  Chin-Hsiang CHANG ,  Jenping KU ,  Ming-Tsung YANG

IPC: C12M1/42 ,  C12M1/00 ,  C12M1/12 ,  C12M3/06

Abstract: A microfluidic EP device for exogenous molecules transfection is disclosed that has high speed, high viability, and efficiency for collection of cells after EP. The microfluidic EP device has an EP chamber assembly, an adaptor, a pop up device, a syringe pump assembly, an EP controller, and a system controller. The EP chamber assembly has a MEMS nano channel plate, a MEMS cap, a cell cavity plate, and a cell cavity plate holder. The EP chamber assembly is connected to the pop up device through the adaptor. The pop up device may be an ultrasound vibrator or a motorized rotator. The MEMS cap has inlets/outlets for inputting/outputting cell solution, washing solution, transfected cells, exogenous material solution. The solution fluid is inputted/outputted by plastic tube and needle adaptor to the syringe pump assembly. All operation sequences are controlled by the system controller, which may perform batch operation continuously.