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公开(公告)号：US20250043227A1
公开(公告)日：2025-02-06
申请号：US18362312
申请日：2023-07-31
Applicant: ORIENTAL SYSTEM TECHNOLOGY INC.
Inventor： Chein-Hsun WANG , Yun-Hsiang CHEN , Jyh-Yih LEU , Wen-Chie HUANG , Chin-Hsiang CHANG , Jenping KU , Ming-Tsung YANG
Abstract: A microfluidic EP device for exogenous molecules transfection is disclosed that has high speed, high viability, and efficiency for collection of cells after EP. The microfluidic EP device has an EP chamber assembly, an adaptor, a pop up device, a syringe pump assembly, an EP controller, and a system controller. The EP chamber assembly has a MEMS nano channel plate, a MEMS cap, a cell cavity plate, and a cell cavity plate holder. The EP chamber assembly is connected to the pop up device through the adaptor. The pop up device may be an ultrasound vibrator or a motorized rotator. The MEMS cap has inlets/outlets for inputting/outputting cell solution, washing solution, transfected cells, exogenous material solution. The solution fluid is inputted/outputted by plastic tube and needle adaptor to the syringe pump assembly. All operation sequences are controlled by the system controller, which may perform batch operation continuously.
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公开(公告)号：US20240327774A1
公开(公告)日：2024-10-03
申请号：US18128441
申请日：2023-03-30
Applicant: ORIENTAL SYSTEM TECHNOLOGY INC.
Inventor： Wen-Chie HUANG , Chein-Hsun WANG , Y. H. CHEN , Chin-Hsiang CHANG , Jenping KU , Yu-Chih LIANG
CPC classification number: C12M35/02 , B01L3/502761 , C12M23/16 , B01L2200/0663 , B01L2300/0681 , B01L2400/0439 , B01L2400/06
Abstract: A microfluidic electroporation device for exogenous molecules transfection is disclosed. The microfluidic electroporation device includes an electroporation chamber assembly, an ultrasound vibrator, and a controller. The electroporation chamber assembly includes an input chamber for exogenous molecules, a MEMS filter, an activation chamber and a MEMS plate. The MEMS plate holds cells within individual cavity for electroporation. Both the MEMS filter and the MEMS plate are made of semiconductor process by wet etching and/or ICP dry etching with V-shaped cavities. The top surfaces of the MEMS filter and the MEMS plate are coated with metal layer for applying electric field during the electroporation process. The electroporation chamber assembly is attached to an ultrasound vibrator which is operated intermittently to allow cells to be fixed in the cavity of the MEMS plate during electroporation process and popped out for collection after electroporation process.
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公开(公告)号：US20240326046A1
公开(公告)日：2024-10-03
申请号：US18127011
申请日：2023-03-28
Applicant: ORIENTAL SYSTEM TECHNOLOGY INC.
Inventor： Wen-Chie HUANG , Chein-Hsun WANG , Y. H. CHEN , Chin-Hsiang CHANG , Jenping KU , Yu-Chih LIANG
CPC classification number: B01L3/502753 , B01D35/06 , B01D35/1573 , B01D35/20 , B01D39/2068 , B01D63/088 , B01D71/0213 , B01D2313/18 , B01L2200/0652 , B01L2300/0681 , B01L2400/0439
Abstract: A microfluidic purification device for exosomes purification is disclosed that has high speed, high viability and efficiency plus re-cycling of cells for regrowth of exosomes. The microfluidic purification device contains coarse filtering, and/or medium filtering plus fine filter with the medium/fine filter made of MEMS semiconductor process. For high-speed operation, an ultrasound vibrator attached to input chamber/filter/output chamber assembly is also used that the vibration amplitude, duty cycle and duration can be controlled through controller. The MEMS filter is V-shaped and/or funnel shape made of silicon wafer by semiconductor process. For funnel shape MEMS filter, the exit hole size is between 0.2 μm to 1 μm suitable for exosomes filtering with high speed and high viability.
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