公开(公告)号：US20190381182A1

公开(公告)日：2019-12-19

申请号：US16480792

申请日：2018-02-01

Applicant: PFIZER INC.

Inventor： Kaushik DUTTA ,  Jose Manuel GOMES ,  Frank W. KOTCH ,  Vimalkumar B. PATEL ,  Amarnauth Shastrie PRASHAD ,  Renee L. PROCOPIO-MELINO ,  Xiaotian ZHONG

IPC: A61K47/68 ,  C07K16/30

Abstract: Optimizing production of selectively capped, and uncapped, cysteines on antibodies by manipulation of cell growth conditions including the deliberate depletion of cysteine and/or cystine in the cell culture process by way of media components, batch duration, or cell density to achieve efficient production of proteins including antibody-drug-conjugates (ADCs); conjugating a TNB-capped cysteine-containing protein by reacting it with a reducing agent capable of detaching the TNB-capping moieties from the protein without significantly reducing antibody inter-chain sulfur bonds, and conjugating reduced sulfur bonds on the protein to a payload through a reactive linking moiety.

公开(公告)号：US20240117030A1

公开(公告)日：2024-04-11

申请号：US18175796

申请日：2023-02-28

Applicant: Pfizer Inc.

Inventor： Rita Diane AGOSTINELLI ,  James Reasoner APGAR ,  Eric Matthew BENNETT ,  Laird BLOOM ,  Ting CHEN ,  Aaron Michael D'ANTONA ,  Arnab DE ,  Fang JIN ,  Marion Teresa KASAIAN ,  Matthew Allister LAMBERT ,  Kimberly Ann MARQUETTE ,  Virginie MCMANUS ,  Jessica Haewon MIN DEBARTOLO ,  Nicole Melissa PICHE-NICHOLAS ,  Richard Thomas SHELDON ,  Lioudmila TCHISTIAKOVA ,  Alexander Michael Shuford BARRON ,  Richard Lee GIESECK, III ,  Xiaotian ZHONG

IPC: C07K16/24 ,  A61K45/06 ,  A61P35/00

CPC classification number: C07K16/247 ,  A61K45/06 ,  A61P35/00 ,  A61K2039/505

Abstract: The present invention relates to antibodies that specifically bind to one or more of IL-4, IL-13, IL-33, TSLP, and p40. The present invention further relates to antibodies that bind to one of IL-4, IL-13, IL-33, or TSLP. The invention further relates to multispecific antibodies that specifically bind to IL-4 and IL-13, and at least one other target. The present invention relates to multispecific antibodies that bind IL-4, IL-13, and one of IL-33, TSLP, or p40. The present invention also pertains to related molecules, e.g. nucleic acids which encode such antibodies or multispecific antibodies, compositions, and related methods, e.g., methods for producing and purifying such antibodies and multispecific antibodies, and their use in diagnostics and therapeutics.