公开(公告)号：US20190381182A1

公开(公告)日：2019-12-19

申请号：US16480792

申请日：2018-02-01

Applicant: PFIZER INC.

Inventor： Kaushik DUTTA ,  Jose Manuel GOMES ,  Frank W. KOTCH ,  Vimalkumar B. PATEL ,  Amarnauth Shastrie PRASHAD ,  Renee L. PROCOPIO-MELINO ,  Xiaotian ZHONG

IPC: A61K47/68 ,  C07K16/30

Abstract: Optimizing production of selectively capped, and uncapped, cysteines on antibodies by manipulation of cell growth conditions including the deliberate depletion of cysteine and/or cystine in the cell culture process by way of media components, batch duration, or cell density to achieve efficient production of proteins including antibody-drug-conjugates (ADCs); conjugating a TNB-capped cysteine-containing protein by reacting it with a reducing agent capable of detaching the TNB-capping moieties from the protein without significantly reducing antibody inter-chain sulfur bonds, and conjugating reduced sulfur bonds on the protein to a payload through a reactive linking moiety.