公开(公告)号：US20230203460A1

公开(公告)日：2023-06-29

申请号：US18068739

申请日：2022-12-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Christian Helmut Bell ,  Harald Sobek ,  Heiko Walch ,  Kiyoshi Yasukawa ,  Misato Baba

IPC: C12N9/12 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C12N9/1276 ,  C12Q1/68 ,  C12Y207/07049 ,  C12P19/34

Abstract: The present invention relates to a mutant reverse transcriptase (RT) with increased thermal stability relative to the wildtype, a nucleic acid encoding the mutant RT, a cell comprising the mutant RT or the nucleic acid, a kit comprising the mutant RT, the use of the mutant RT for cDNA synthesis, method for reverse transcription of RNA comprising synthesizing cDNA with the use of the mutant RT and a method for detecting an RNA marker in a sample with the use of the mutant RT.

公开(公告)号：US20200040316A1

公开(公告)日：2020-02-06

申请号：US16599407

申请日：2019-10-11

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Christian Helmut Bell ,  Harald Sobek ,  Heiko Walch ,  Kiyoshi Yasukawa ,  Misato Baba

IPC: C12N9/12 ,  C12Q1/68

Abstract: The present invention relates to a mutant reverse transcriptase (RT) with increased thermal stability relative to the wildtype, a nucleic acid encoding the mutant RT, a cell comprising the mutant RT or the nucleic acid, a kit comprising the mutant RT, the use of the mutant RT for cDNA synthesis, method for reverse transcription of RNA comprising synthesizing cDNA with the use of the mutant RT and a method for detecting an RNA marker in a sample with the use of the mutant RT.

公开(公告)号：US12234488B2

公开(公告)日：2025-02-25

申请号：US18068739

申请日：2022-12-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Christian Helmut Bell ,  Harald Sobek ,  Heiko Walch ,  Kiyoshi Yasukawa ,  Misato Baba

IPC: C12P19/34 ,  C12N9/12 ,  C12Q1/68

Abstract: The present invention relates to a mutant reverse transcriptase (RT) with increased thermal stability relative to the wildtype, a nucleic acid encoding the mutant RT, a cell comprising the mutant RT or the nucleic acid, a kit comprising the mutant RT, the use of the mutant RT for cDNA synthesis, method for reverse transcription of RNA comprising synthesizing cDNA with the use of the mutant RT and a method for detecting an RNA marker in a sample with the use of the mutant RT.

公开(公告)号：US11618892B2

公开(公告)日：2023-04-04

申请号：US16599407

申请日：2019-10-11

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Christian Helmut Bell ,  Harald Sobek ,  Heiko Walch ,  Kiyoshi Yasukawa ,  Misato Baba

IPC: A61K38/00 ,  C12N9/12 ,  C12Q1/68 ,  C12P19/34

Abstract: The present invention relates to a mutant reverse transcriptase (RT) with increased thermal stability relative to the wildtype, a nucleic acid encoding the mutant RT, a cell comprising the mutant RT or the nucleic acid, a kit comprising the mutant RT, the use of the mutant RT for cDNA synthesis, method for reverse transcription of RNA comprising synthesizing cDNA with the use of the mutant RT and a method for detecting an RNA marker in a sample with the use of the mutant RT.