METHOD AND APPARATUS FOR PERFORMING QUANTITATIVE ANALYSIS OF NUCLEIC ACID USING REAL-TIME PCR
    2.
    发明申请
    METHOD AND APPARATUS FOR PERFORMING QUANTITATIVE ANALYSIS OF NUCLEIC ACID USING REAL-TIME PCR 审中-公开
    使用实时PCR进行核酸定量分析的方法和装置

    公开(公告)号:US20140005948A1

    公开(公告)日:2014-01-02

    申请号:US13846456

    申请日:2013-03-18

    CPC classification number: G01N33/48 C12Q1/6851 C12Q2537/165

    Abstract: A method and apparatus for performing quantitative analysis of a nucleic acid by determining a curve-fitting area based on fluorescence intensity data obtained by performing PCR on a target nucleic acid; analyzing parameters for amplification efficiency and nucleic acid concentration by curve-fitting a result of performing PCR on a reference nucleic acid with a known initial nucleic acid concentration; and estimating the initial nucleic acid concentration of the target nucleic acid by performing curve-fitting on the determined curve-fitting area using the analyzed parameters.

    Abstract translation: 一种用于通过基于通过对靶核酸进行PCR得到的荧光强度数据确定曲线拟合面积来进行核酸定量分析的方法和装置; 通过对具有已知初始核酸浓度的参照核酸进行PCR的结果曲线拟合分析扩增效率和核酸浓度的参数; 以及通过使用所分析的参数在确定的曲线拟合区域上进行曲线拟合来估计靶核酸的初始核酸浓度。

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