公开(公告)号：US20200318050A1

公开(公告)日：2020-10-08

申请号：US16838511

申请日：2020-04-02

Applicant: SOUTH DAKOTA BOARD OF REGENTS

Inventor： SCOTT WOOD ,  RAM SARASWAT

IPC: C12M1/12 ,  C12N5/077

Abstract: The present disclosure describes hydrogels which are micropatterned with a network of wells for cell culture. In a preferred embodiment, the micropatterned hydrogels are embedded with a nanomaterial. Further described are methods of forming the micropatterned hydrogels and methods of culturing cells in the micropatterned hydrogels. The hydrogels can be natural or synthetic.

公开(公告)号：US20240043779A1

公开(公告)日：2024-02-08

申请号：US18487600

申请日：2023-10-16

Applicant: SOUTH DAKOTA BOARD OF REGENTS

Inventor： SCOTT WOOD ,  RAM SARASWAT

IPC: C12M3/06 ,  B01L3/00 ,  C12M1/00 ,  C12M1/12

CPC classification number: C12M23/16 ,  B01L3/502715 ,  C12M23/22 ,  C12M25/14

Abstract: A platform for culturing modular, biomimetic compositions such as tissues, cartilage, bone, synovial membrane, is accomplished through the use of a 3D printed platform with cell well, well plate frame with culture and analysis modules, coverglass bottoms for imaging, and cross-talk flow to connect tissue modules for paracrine signaling. Human chondrocytes can be generated and kept in a cell back and expanded to zonal models, osteoarthritis progression models. The use of titanium oxide nanotubes and can produce bone marrow stem cells differentiated toward osteoblasts. The synovial membrane can be modeled by an electrospun mesh, macrophages with an inducible phenotype (quiescent vs. wound repair vs. inflammatory).