公开(公告)号：US20210071122A1

公开(公告)日：2021-03-11

申请号：US16960239

申请日：2018-04-04

Applicant: SOUTHEAST UNIVERSITY

Inventor： Zhen ZHU ,  Qiaodan LI ,  Dingxin JIN ,  Feng YU ,  Yangye GENG

IPC: C12M3/00 ,  C12M3/06 ,  C12M1/34 ,  B01L3/00

Abstract: A microfluidic chip for culturing and real-time monitoring of multicellular tissues and use method thereof. The chip comprises a glass substrate layer, and a PDMS microchannel layer located on the glass substrate layer, wherein the glass substrate layer comprises a glass substrate, and a plurality of microelectrodes thereon; the PDMS microchannel comprises a plurality of independent microfluidic channels; the microelectrodes on the glass substrate are in one-to-one correspondence with the microfluidic channels in the PDMS microchannel layer; and the microelectrodes are electrically connected to an external circuit. The use method comprises: cell capture, cell or tissue culture, electrical impedance spectroscopy detection, and tissue release.