公开(公告)号：US20250145970A1

公开(公告)日：2025-05-08

申请号：US18933327

申请日：2024-10-31

Applicant: Shandong University of Technology

Inventor： Xiuzhen Gao ,  Qinyuan Ma ,  Yanmiao Yin ,  Yanling Zhao ,  Zhixin Ma ,  Tong Zhang

IPC: C12N9/04 ,  C12P7/62

Abstract: The present disclosure relates to the technical field of biotechnology, in particular to carbonyl reductase mutant and its application. Specifically, the present disclosure provides a carbonyl reductase mutant for catalyzing the preparation of (R)-8-chloro-6-hydroxyoctanoic acid ethyl ester from 8-chloro-6-oxooctanoic acid ethyl ester. The mutant protein is a non natural protein and undergoes mutations in three core amino acids related to enzyme catalytic activity in the initial carbonyl reductase. The advantageous effect of the present disclosure is that the carbonyl reductase mutant provided by the present disclosure has the characteristic of efficiently catalyzing the preparation of the precursor substance (R)-8-chloro-6-hydroxyoctanoic acid from 8-chloro-6-oxooctanoic acid ethyl ester, and can efficiently catalyze the oxidation-reduction reaction of 8-chloro-6-oxooctanoic acid, with a product space-time yield of 99.9 g·L−1·h−1 or more.