公开(公告)号：US20100124533A1

公开(公告)日：2010-05-20

申请号：US12274817

申请日：2008-11-20

申请人： Stefan Tunev ,  Ankit Shah ,  Alejandra Caceres ,  Ya Guo ,  Julie Trudel ,  Phean Him ,  Ayala Hezi-Yamit

发明人： Stefan Tunev ,  Ankit Shah ,  Alejandra Caceres ,  Ya Guo ,  Julie Trudel ,  Phean Him ,  Ayala Hezi-Yamit

IPC分类号： A61B10/00 ,  A01K67/00

CPC分类号： A01K67/027 ,  A01K2217/00 ,  A01K2227/108 ,  A01K2267/03 ,  A01K2267/0375

摘要： An animal model for cardiovascular disease comprising one or more vascular plaque lesions formed at selected sites within a vascular segment of a nonhuman mammal. The vascular plaque lesion is formed by administering a hypercholesterolemic diet to the nonhuman mammal, inflicting an injury to the vascular wall at the selected site after a predetermined exposure to the hypercholesterolemic diet, and applying a hydrogel to the injured vascular wall. Another aspect of the invention provides a method for evaluating a test compound for an effect on atherosclerotic lesion formation comprising administering to a nonhuman mammal a hypercholesterolemic diet, and, after a defined period of time, isolating a segment of a blood vessel using a balloon catheter, inflicting an injury to the vascular wall within the isolated segment, and applying a hydrogel within the vascular segment. The method further comprises forming a vascular plaque lesion on the vascular wall at the site of the injury, delivering the test compound to the nonhuman mammal, and monitoring atherosclerotic lesion size and composition at the injured site after a defined period of exposure to the test compound.

摘要翻译： 一种用于心血管疾病的动物模型，其包含在非人哺乳动物的血管片段内的选定位置处形成的一个或多个血管斑块损伤。 通过向非人哺乳动物施用高胆固醇血症饮食来形成血管斑块损伤，在预先暴露于高胆固醇血症饮食之后对选定部位的血管壁施加损伤，并将水凝胶施加到损伤的血管壁。 本发明的另一方面提供了一种用于评估对动脉粥样硬化病变形成的影响的测试化合物的方法，包括向非人类哺乳动物施用高胆固醇血症饮食，并且在限定的时间段之后，使用气囊导管分离血管的一段 对隔离区段内的血管壁造成损伤，并在血管段内施用水凝胶。 该方法还包括在损伤部位的血管壁上形成血管斑块损伤，将测试化合物递送至非人类哺乳动物，并在暴露于测试化合物的确定的时间段后监测受伤部位的动脉粥样硬化病变大小和组成 。