公开(公告)号：US20240050566A1

公开(公告)日：2024-02-15

申请号：US18361787

申请日：2023-07-28

Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED

Inventor： Shuxia ZHOU ,  Lan Cao ,  Dayong Gao ,  Qiong Xue ,  Jiusong Sun ,  Huang Zhu

IPC: A61K39/00 ,  A01N1/02 ,  C12N5/0783 ,  A61P35/00

CPC classification number: A61K39/4613 ,  A01N1/0284 ,  C12N5/0646 ,  A61K39/4631 ,  A61P35/00 ,  C12N2510/00 ,  A61K2239/13 ,  A61K2239/21 ,  A61K2239/25

Abstract: The present disclosure provides, among other things, a method of cryopreserving and thawing cells that results in the thawed cells having high cellular viability and functionality post-thawing. In some embodiments, a large-scale method of cryopreserving cells is provided, the method comprising: (a) contacting the cells with a cryopreservation medium; (b) cooling the cells to −80° C. at a controlled rate to minimize latent heat of fusion; and (c) storing the cells in liquid nitrogen vapor phase, thereby cryopreserving the immune cells.