公开(公告)号：US20240050566A1

公开(公告)日：2024-02-15

申请号：US18361787

申请日：2023-07-28

Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED

Inventor： Shuxia ZHOU ,  Lan Cao ,  Dayong Gao ,  Qiong Xue ,  Jiusong Sun ,  Huang Zhu

IPC: A61K39/00 ,  A01N1/02 ,  C12N5/0783 ,  A61P35/00

CPC classification number: A61K39/4613 ,  A01N1/0284 ,  C12N5/0646 ,  A61K39/4631 ,  A61P35/00 ,  C12N2510/00 ,  A61K2239/13 ,  A61K2239/21 ,  A61K2239/25

Abstract: The present disclosure provides, among other things, a method of cryopreserving and thawing cells that results in the thawed cells having high cellular viability and functionality post-thawing. In some embodiments, a large-scale method of cryopreserving cells is provided, the method comprising: (a) contacting the cells with a cryopreservation medium; (b) cooling the cells to −80° C. at a controlled rate to minimize latent heat of fusion; and (c) storing the cells in liquid nitrogen vapor phase, thereby cryopreserving the immune cells.

公开(公告)号：US20250031688A1

公开(公告)日：2025-01-30

申请号：US18361790

申请日：2023-07-28

Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED

Inventor： Shuxia ZHOU ,  Yana WANG ,  Lan CAO ,  Dayong GAO ,  Qiong XUE ,  Jiusong SUN ,  Huang ZHU

IPC: A01N1/02 ,  A61K39/00 ,  C07K14/54 ,  C07K14/705 ,  C07K14/725 ,  C07K16/28

Abstract: The present disclosure provides, among other things, a cryopreservation medium comprising a cryoprotectant, an albumin, a disaccharide and a non-pyrogenic and isotonic crystalloid solution. The disclosure also provides, among other things, a cryopreservation medium for cryopreserving immune cells, the medium comprising: human serum albumin (HSA), sodium chloride, sodium gluconate, sodium acetate trihydrate, potassium chloride, magnesium chloride, dimethyl sulfoxide (DMSO), and a trehalose. The present disclosure also provides, a method of cryopreserving immune cells, transporting and subsequently administering such immune cells to a patient in need thereof.

公开(公告)号：US20240306632A1

公开(公告)日：2024-09-19

申请号：US18274988

申请日：2022-02-09

Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED

Inventor： Shuxia ZHOU ,  Yana WANG ,  Lan CAO ,  Dayong GAO ,  Qiong XUE ,  Jiusong SUN ,  Huang ZHU

IPC: A01N1/02

CPC classification number: A01N1/0221 ,  A01N1/0226

Abstract: The present disclosure provides, among other things, a cryopreservation medium comprising a cryoprotectant, an albumin, a disaccharide and a non-pyrogenic and isotonic crystalloid solution. The disclosure also provides, among other things, a cryopreservation medium for cryopreserving immune cells, the medium comprising: human serum albumin (HSA), sodium chloride, sodium gluconate, sodium acetate trihydrate, potassium chloride, magnesium chloride, dimethyl sulfoxide (DMSO), and a trehalose. The present disclosure also provides, a method of cryopreserving immune cells, transporting and subsequently administering such immune cells to a patient in need thereof.