公开(公告)号：US20170216457A1

公开(公告)日：2017-08-03

申请号：US15514889

申请日：2015-10-01

Applicant: UNIVERSITY OF SEOUL INDUSTRY COOPERATION FOUNDATION

Inventor： Jong-Bum LEE ,  Hye-Jin KIM

IPC: A61K48/00 ,  C12N15/63 ,  A61K9/14 ,  C12P19/34

CPC classification number: A61K48/0066 ,  A61K9/14 ,  A61K48/0016 ,  A61K48/0091 ,  B82B1/00 ,  B82Y5/00 ,  C12N15/63 ,  C12N15/87 ,  C12P19/34 ,  C12Y207/07006

Abstract: Disclosed are nanoparticles that are introduced into cells and express a specific protein and a manufacturing method thereof. More particularly, the present invention relates to mRNA nanoparticles, which increase the expression of a specific protein capable of stimulating the cellular immune system to induce cellular immune responses and are thus applicable to treat a variety of diseases, do not require passage across the nuclear envelope because a desired gene is delivered not as plasmid DNA itself but in the form of mRNA, thus improving the efficiency of protein expression, and the nanoparticles are generated through a one-step process with a relatively small amount of plasmid DNA via rolling circle transcription (RCT), thereby providing a simple and economical process for gene delivery. The present invention is also concerned with such mRNA nanoparticles.

公开(公告)号：US20210388322A1

公开(公告)日：2021-12-16

申请号：US17288470

申请日：2018-10-29

Applicant: UNIVERSITY OF SEOUL INDUSTRY COOPERATION FOUNDATION

Inventor： Jong-Bum LEE ,  Hye-Jin KIM ,  Ju-Hyun PARK

IPC: C12N5/074 ,  C12N15/87 ,  C12N15/113

Abstract: The present invention pertains to a method for producing induced pluripotent stem cells, and more specifically, to a method for producing induced pluripotent stem cells using RNA nanoparticles for cell transformation, wherein: cell transformation can be effectively performed without genetic modification by producing induced pluripotent stem cells using self-assembled RNA nanoparticles including at least one RNA selected from the group consisting of messenger RNA for expressing transcription factors which induce somatic cells and adult stem cells to be dedifferentiated into induced pluripotent stem cells, micro RNA facilitating the dedifferentiation process, and small interfering RNA; the production efficiency of iPSCs can be maximized by adjusting structural properties and activity; and low gene loading efficiency can be overcome by applying an infinite replication process to incorporate high concentrations of RNA in RNA nanoparticles.