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公开(公告)号：US20170216457A1
公开(公告)日：2017-08-03
申请号：US15514889
申请日：2015-10-01
Inventor： Jong-Bum LEE , Hye-Jin KIM
CPC classification number: A61K48/0066 , A61K9/14 , A61K48/0016 , A61K48/0091 , B82B1/00 , B82Y5/00 , C12N15/63 , C12N15/87 , C12P19/34 , C12Y207/07006
Abstract: Disclosed are nanoparticles that are introduced into cells and express a specific protein and a manufacturing method thereof. More particularly, the present invention relates to mRNA nanoparticles, which increase the expression of a specific protein capable of stimulating the cellular immune system to induce cellular immune responses and are thus applicable to treat a variety of diseases, do not require passage across the nuclear envelope because a desired gene is delivered not as plasmid DNA itself but in the form of mRNA, thus improving the efficiency of protein expression, and the nanoparticles are generated through a one-step process with a relatively small amount of plasmid DNA via rolling circle transcription (RCT), thereby providing a simple and economical process for gene delivery. The present invention is also concerned with such mRNA nanoparticles.
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Patent Agency Ranking
2.发明申请公开(公告)号：US20200224193A1
公开(公告)日：2020-07-16
申请号：US16596553
申请日：2019-10-08
Inventor： Jong-Bum LEE , Hye-Jin Kim
Abstract: The present invention relates to a method of manufacturing vesicles by delivery of RNA nanoparticles, in which messenger RNA nanoparticles for target protein expression are delivered to a cell, and vesicles manufactured using the same. A protein is locally over-expressed in the cell to thus be excreted through the vesicles to the outside of the cell, which enables the vesicles containing a target protein to be easily mass-produced. The vesicles containing the target protein is obtained regardless of the cell type. The concentration of the messenger RNA nanoparticles delivered to the cell is adjusted, thus adjusting the manufacturing amount and the manufacturing time of the vesicles. After the surface of the cell is reformed, the messenger RNA nanoparticles are delivered thereto, thus obtaining the vesicles carrying the target protein and having a surface property of a specific function.
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公开(公告)号：US20190185895A1
公开(公告)日：2019-06-20
申请号：US15849006
申请日：2017-12-20
Inventor： Jong-Bum LEE , Jae-Sung LEE
Abstract: This invention relates to DNA-Mn hybrid particles and a method of manufacturing the same, the method including producing a circular DNA template for replication and forming particles in which DNA and Mn are bound to each other using Mn during the synthesis of a new strand of DNA from the circular DNA template for replication using a DNA polymerase, thus promoting the activity of the DNA polymerase using the coenzyme function of Mn and broadening the range of application fields of DNA as a biomaterial.
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公开(公告)号：US20170240889A1
公开(公告)日：2017-08-24
申请号：US15321748
申请日：2015-06-30
Inventor： Jong-Bum LEE , Yong-Kuk PARK
IPC: C12N15/113 , C12Q1/68 , C12P19/34
CPC classification number: C12N15/113 , C12N2310/14 , C12N2310/16 , C12N2310/532 , C12N2320/32 , C12P19/34 , C12Q1/6844 , C12Q1/6853 , C12Q1/6867 , C12Q2525/205 , C12Q2525/207 , C12Q2525/307 , C12Q2527/113
Abstract: Disclosed are particles which are introduced into target cells and suppress the expression of specific genes, and a method of manufacturing such particles. More particularly, the present invention relates to DNA-RNA hybrid particles that comprise a DNA strand and an RNA strand that binds to the DNA strand through partial complementary base pairing, in which the DNA strand comprises an aptamer sequence that is able to bind to a target protein produced in a target cell, and the RNA strand comprises an siRNA sequence that binds to a target RNA in the target cell to suppress protein expression from the target RNA. Such hybrid particles are capable of effectively delivering an siRNA therapeutic agent into target cells for the treatment of disease, and have resistance against digestion by in vivo nucleases, DNase and RNase, owing to complementary binding formed between DNA and RNA strands. Also, the present invention relates to a method of manufacturing such DNA-RNA hybrid particles.
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5.发明申请公开(公告)号：US20210388322A1
公开(公告)日：2021-12-16
申请号：US17288470
申请日：2018-10-29
Inventor： Jong-Bum LEE , Hye-Jin KIM , Ju-Hyun PARK
IPC: C12N5/074 , C12N15/87 , C12N15/113
Abstract: The present invention pertains to a method for producing induced pluripotent stem cells, and more specifically, to a method for producing induced pluripotent stem cells using RNA nanoparticles for cell transformation, wherein: cell transformation can be effectively performed without genetic modification by producing induced pluripotent stem cells using self-assembled RNA nanoparticles including at least one RNA selected from the group consisting of messenger RNA for expressing transcription factors which induce somatic cells and adult stem cells to be dedifferentiated into induced pluripotent stem cells, micro RNA facilitating the dedifferentiation process, and small interfering RNA; the production efficiency of iPSCs can be maximized by adjusting structural properties and activity; and low gene loading efficiency can be overcome by applying an infinite replication process to incorporate high concentrations of RNA in RNA nanoparticles.
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公开(公告)号：US20160281123A1
公开(公告)日：2016-09-29
申请号：US15035747
申请日：2014-11-18
Inventor： Jong-Bum LEE , Dae-Hoon HAN
CPC classification number: C12P19/34 , C12N15/10 , C12N15/1003
Abstract: Disclosed are a method of manufacturing an RNA membrane and an RNA membrane manufactured thereby, wherein the RNA membrane can be produced at lower cost, in which the RNA membrane is composed exclusively of RNA, and thus has no toxicity in vivo, is controllable, and can be effectively applied to bio-organs such as the pericardium, as well as the production of peptides or proteins, and particularly, long linear RNA strands, which have not yet formed particles, are concentrated on the surface of a tube by inducing an evaporation process to thus activate the bonding of base pairs thereof, and the roughness and thickness of the RNA membrane can be controlled by changing the manufacturing conditions.
Abstract translation: 公开了制造由此制备的RNA膜和RNA膜的方法，其中RNA膜可以以较低的成本生产，其中RNA膜仅由RNA组成，因此在体内没有毒性，是可控的，以及 可以有效地应用于诸如心包膜的生物器官，以及肽或蛋白质的生产，特别是尚未形成颗粒的长线型RNA链通过诱导蒸发而浓缩在管的表面上 从而激活其碱基对的键合，并且可以通过改变制造条件来控制RNA膜的粗糙度和厚度。
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