公开(公告)号：US20230323397A1

公开(公告)日：2023-10-12

申请号：US18100028

申请日：2023-01-23

Applicant: UNIVERSITY OF SOUTH CAROLINA

Inventor： SUJIT PUJHARI ,  ROBERT ANDERSON SMITHWICK

IPC: C12N15/113 ,  C12N9/22 ,  C12N15/86

CPC classification number: C12N15/86 ,  C12N9/22 ,  C12N15/1131 ,  C12N2310/122 ,  C12N2750/14143

Abstract: Disclosed is a method of purifying recombinant adeno-associated virus (rAAV) particles. For instance, the rAAV particles may be derived from an AAV-producing cell culture. According to the present disclosure, the method can include a) producing a crude cell lysate comprising rAAV particles by hypotonic lysing of the cell culture; b) clarifying the crude cell lysate using filtration producing a supernatant comprising rAAV particles; c) isolating the supernatant to obtain a virus pellet comprising rAAV particles; and d) resuspending the virus pellet with a buffer to obtain purified rAAV particles.