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公开(公告)号：US12098353B2
公开(公告)日：2024-09-24
申请号：US18124923
申请日：2023-03-22
发明人： Megan McCain , Joycelyn Yip , Ching-Ling Lien , Michael Harrison
CPC分类号： C12M23/16 , A01N1/0247 , B01L3/502715 , C12M23/22 , C12M23/24 , B01L2200/0684 , B01L2300/087 , B01L2300/0877 , B01L2300/12
摘要： Zebrafish are a powerful model for investigating cardiac repair due to their unique regenerative abilities, scalability, and compatibility with many genetic tools. However, characterizing the regeneration process in live adult zebrafish hearts has proved challenging because adult fish are opaque and explanted hearts in conventional culture conditions experience rapid declines in morphology and physiology. To overcome these limitations, we fabricated a fluidic device for culturing explanted adult zebrafish hearts with constant media perfusion that is also compatible with live imaging. Unlike hearts cultured in dishes for one week, the morphology and calcium activity of hearts cultured in the device for one week were largely similar to freshly explanted hearts. We also cultured injured hearts in the device and used live imaging techniques to continuously record the revascularization process over several days, demonstrating how our device enables unprecedented visual access to the multi-day process of adult zebrafish heart regeneration.
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公开(公告)号：US11629320B2
公开(公告)日：2023-04-18
申请号：US16654489
申请日：2019-10-16
发明人： Megan McCain , Joycelyn Yip , Ching-Ling Lien , Michael Harrison
摘要： Zebrafish are a powerful model for investigating cardiac repair due to their unique regenerative abilities, scalability, and compatibility with many genetic tools. However, characterizing the regeneration process in live adult zebrafish hearts has proved challenging because adult fish are opaque and explanted hearts in conventional culture conditions experience rapid declines in morphology and physiology. To overcome these limitations, we fabricated a fluidic device for culturing explanted adult zebrafish hearts with constant media perfusion that is also compatible with live imaging. Unlike hearts cultured in dishes for one week, the morphology and calcium activity of hearts cultured in the device for one week were largely similar to freshly explanted hearts. We also cultured injured hearts in the device and used live imaging techniques to continuously record the revascularization process over several days, demonstrating how our device enables unprecedented visual access to the multi-day process of adult zebrafish heart regeneration.
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