公开(公告)号：US20220162590A1

公开(公告)日：2022-05-26

申请号：US17546978

申请日：2021-12-09

Applicant: Ultima Genomics, Inc.

Inventor： Gilad ALMOGY ,  Eyal NEISTEIN ,  Mark PRATT

IPC: C12N15/10 ,  G16B20/20 ,  G16B40/20 ,  C12Q1/6869

Abstract: The present disclosure provides methods for accurate base calling of sequences using molecular barcodes. A method for sequencing nucleic acid molecules may comprise: (a) using barcode molecules to barcode nucleic acid molecules from a sample, to generate barcoded nucleic acid molecules comprising barcode sequences; (b) sequencing the barcoded nucleic acid molecules to generate sequencing signals comprising signals corresponding to the barcode sequences, wherein the sequencing signals are not sequencing reads; (c) using the signals corresponding to the barcode sequences to group the sequencing signals into groups, wherein sequencing signals of a given group comprise signals corresponding to a barcode sequence that is (i) identical for the given group and (ii) different from barcode sequences of other groups; (d) processing the sequencing signals within the given group to generate sets of aggregated signals which are not sequencing reads; and (e) combining the sets of aggregated signals to generate a consensus sequence.

公开(公告)号：US20240386998A1

公开(公告)日：2024-11-21

申请号：US18426104

申请日：2024-01-29

Applicant: Ultima Genomics, Inc.

Inventor： Simchon FAIGLER ,  Eyal NEISTEIN ,  Mark PRATT

IPC: G16B30/00 ,  G06T5/20 ,  G06T5/50 ,  G06T7/00 ,  G06T7/194 ,  G06V20/69 ,  G06V20/70

Abstract: The present disclosure relates generally to sequencing techniques, and more specifically to methods, systems, devices, and non-transitory computer-readable storage media for processing images of biological samples (e.g., to obtain sequencing data). An exemplary method of determining nucleic acid sequences of a plurality of sequencing colonies comprises: obtaining an input image of a surface, wherein the plurality of sequencing colonies are attached to the surface; detecting a set of sequencing colonies of the plurality of sequencing colonies in the input image; executing in parallel, using a graphics processor, a plurality of iterative processes to obtain signal amplitudes for the detected set of sequencing colonies, wherein each iterative process corresponds to a respective detected sequencing colony in the set; and determining, at least partially based on the signal amplitudes for the detected set of sequencing colonies, portions of nucleic acid sequences of the plurality of sequencing colonies.