公开(公告)号：US20130102049A1

公开(公告)日：2013-04-25

申请号：US13548181

申请日：2012-07-12

Applicant: Wen-Bin YANG

Inventor： Wen-Bin YANG

IPC: G01N33/58 ,  C07H15/26

CPC classification number: G01N33/58 ,  C07H15/26 ,  G01N33/531 ,  Y10T436/14

Abstract: Novel method and reagents for generating reversibly tagged saccharides, aldehydes, carboxyl acids, or orthoacetates useful in analytical and diagnostic applications are disclosed. Saccharides are coupled at the reducing end to tagging moieties comprising a reagent selected from a ortho-diaminobenzoic(DAB)-peptide, an aldo-imidazole or N-methylated aldo-imidazole, or an ortho-phenyldiamine (OPD) or substituted OPD. The tagged saccharide further comprising detectable or functional groups coupled to the tagging moiety are provided. Kits and reagents for chromatography and mass spectrometry are disclosed.

Abstract translation: 公开了用于生成可用于分析和诊断应用的可逆标记的糖，醛，羧酸或原乙酸盐的新方法和试剂。 糖在还原端耦合到包含选自邻二氨基苯甲酸（DAB）肽，醛 - 咪唑或N-甲基化的醛 - 咪唑或邻苯基二胺（OPD）或取代的OPD的试剂的标记部分。 提供了进一步包含与标记部分偶联的可检测或官能团的标记糖。 公开了用于色谱和质谱的试剂盒和试剂。