利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

288 条记录 (耗时 0.039 秒)

    Analyte detection method
    2.
    发明授权
    Analyte detection method 有权
    分析物检测方法

    公开(公告)号：US09068996B2

    公开(公告)日：2015-06-30

    申请号：US13878815

    申请日：2011-10-11

    申请人： David Michael Pettigrew ,  Peter Georg Laitenberger ,  Bo Liu

    发明人： David Michael Pettigrew ,  Peter Georg Laitenberger ,  Bo Liu

    IPC分类号： G01N33/94 ,  G01N33/50 ,  G01N33/52 ,  G01N33/557

    CPC分类号： G01N33/948 ,  G01N33/52 ,  G01N33/557 ,  Y10T436/14 ,  Y10T436/203332

    摘要： Disclosed is a method of determining the presence of an analyte of interest by means of a detection of a reaction product between the analyte of interest and a reactant, the method comprising extracting the analyte of interest from a complex sample matrix; transferring the analyte of interest to an initial reaction mixture; performing a background measurement on the initial reaction mixture comprising at most a negligible concentration of the reaction product, wherein the reaction conditions present in said initial reaction mixture at least reduce the reaction rate of the formation of the reaction product such that the background measurement can be performed without a measurable change in said negligible concentration; altering the reaction conditions in the initial reaction mixture to accelerate said reaction rate; continuing said reaction until the concentration of the reaction product has stabilized; performing a second measurement on the resultant reaction mixture to obtain a signal correlated to said concentration; and determining the presence of the analyte of interest from a difference between the background measurement and the second measurement. In a preferred embodiment, the analyte of interest is Propofol (2,6-di-isopropylphenol), and the reactant is the activated Gibbs reagent (2,6-dichloroquinoneimine).

    摘要翻译： 公开了通过检测目标分析物和反应物之间的反应产物来确定感兴趣分析物的存在的方法，该方法包括从复杂样品基质提取目的分析物; 将目标分析物转移到初始反应混合物中; 对最初反应混合物进行背景测量，该反应混合物至少含有可忽略的反应产物浓度，其中存在于所述初始反应混合物中的反应条件至少降低反应产物形成的反应速率，使得背景测量可以 在所述可忽略的浓度中没有可测量的变化; 改变初始反应混合物中的反应条件以加速所述反应速率; 继续反应直到反应产物的浓度稳定; 对所得反应混合物进行第二次测定以获得与所述浓度相关的信号; 以及从背景测量和第二测量之间的差异确定感兴趣分析物的存在。 在优选的实施方案中，感兴趣的分析物是异丙酚（2,6-二异丙基苯酚），反应物是活化的吉布斯试剂（2,6-二氯喹啉亚胺）。

    Optical reporter compositions
    3.
    发明授权
    Optical reporter compositions 有权
    光学记者组合物

    公开(公告)号：US09005985B2

    公开(公告)日：2015-04-14

    申请号：US13761447

    申请日：2013-02-07

    申请人： APDN (B.V.I.) Inc.

    发明人： Thomas Kwok ,  Benjamin MingHwa Liang ,  Stephane Shu Kin So

    IPC分类号： C12Q1/68 ,  C09D7/12 ,  C09D11/03 ,  C12Q1/00 ,  C08K5/09 ,  B82Y15/00

    CPC分类号： C12Q1/6876 ,  B82Y15/00 ,  C08K5/09 ,  C09D7/63 ,  C09D11/03 ,  C12Q1/6816 ,  Y10S977/774 ,  Y10S977/92 ,  Y10T436/14 ,  Y10T436/143333 ,  C12Q2563/185

    摘要： This invention provides compositions that have a light emitting reporter linked to biomolecules, preferably, nucleotide oligomers. The light reporter particles are silylated and functionalized to produce a coated light reporter particle, prior to covalently linking the biomolecules to the light reporter particle. The light reporter particles of the invention can be excited by a light excitation source such as UV or IR light, and when the biomolecule is DNA, the attached DNA molecule(s) are detectable by amplification techniques such as PCR.

    摘要翻译： 本发明提供具有与生物分子连接的发光报道基团的组合物，优选核苷酸寡聚体。 在将生物分子共价连接到光报告颗粒之前，光报告颗粒被甲硅烷基化和官能化以产生包被的光报告颗粒。 本发明的光报告颗粒可以通过诸如UV或IR光的光激发源激发，并且当生物分子是DNA时，通过诸如PCR的扩增技术检测所连接的DNA分子。

    High purity heparin and production method therefor
    6.
    发明授权
    High purity heparin and production method therefor 有权
    高纯度肝素及其制备方法

    公开(公告)号：US08932867B2

    公开(公告)日：2015-01-13

    申请号：US13822825

    申请日：2011-09-13

    申请人： Michio Muguruma ,  Hiroshi Murata

    发明人： Michio Muguruma ,  Hiroshi Murata

    IPC分类号： C08B37/00 ,  A61K31/727 ,  G01N30/14

    CPC分类号： C08B37/0075 ,  A61K31/727 ,  C08B37/0003 ,  C08B37/0078 ,  G01N30/14 ,  Y10T436/14 ,  Y10T436/142222 ,  Y10T436/143333

    摘要： The present invention provides a high purity heparin useful to be a pharmaceutical product, cosmetics, research reagent, or the like, and a method for producing the same, more specifically, a heparin which does not substantially contain a nitrous acid degradation-resistant impurity and a method for producing a heparin, comprising mixing an aqueous solution of 5 to 30% by weight of the heparin with ethanol having an amount (volume) 0.2 to 1 times the amount (volume) of the aqueous heparin solution to obtain a colloidal precipitate of heparin.

    摘要翻译： 本发明提供了可用作药物，化妆品，研究试剂等的高纯度肝素及其制造方法，更具体而言，是基本上不含有亚硝酸降解性杂质的肝素， 一种肝素的制造方法，其特征在于，将5〜30重量％的肝素水溶液与乙醇配合（体积）为肝素水溶液的量（体积）的0.2〜1倍，得到胶体沉淀物 肝素。

    Detection of nicotine metabolites
    7.
    发明授权
    Detection of nicotine metabolites 有权
    检测尼古丁代谢物

    公开(公告)号：US08785205B2

    公开(公告)日：2014-07-22

    申请号：US13697446

    申请日：2011-05-13

    申请人： Charles John Knill ,  John Frederick Kennedy

    发明人： Charles John Knill ,  John Frederick Kennedy

    IPC分类号： G01N33/52 ,  G01N33/50 ,  G01N33/00 ,  G01N33/94

    CPC分类号： G01N33/52 ,  G01N33/94 ,  Y10T436/14 ,  Y10T436/145555

    摘要： The present invention relates to assays for detection of nicotine metabolites, in particular cotinine, in fluid samples and uses of these assays in quantification of smoking habits. The assays comprise contacting a body fluid sample with a cyanogen halide and a pyrazolone compound and detecting a change in light absorbance of the pyrazolone compound which is associated with the presence of nicotine metabolites. Also provided are assay kits including a cyanogen halide or cyanogen halide-precursor(s) and a pyrazolone compound.

    摘要翻译： 本发明涉及在流体样品中检测尼古丁代谢物，特别是可替宁，并且这些测定在量化吸烟习惯中的用途。 测定法包括使体液样品与氰卤化物和吡唑啉酮化合物接触并检测与存在尼古丁代谢物相关的吡唑啉酮化合物的吸光度变化。 还提供了包括卤化氰或卤化氰前体和吡唑啉酮化合物的测定试剂盒。

    On-chip laboratory for blood analysis
    8.
    发明授权
    On-chip laboratory for blood analysis 失效
    芯片实验室进行血液分析

    公开(公告)号：US08703499B2

    公开(公告)日：2014-04-22

    申请号：US13265334

    申请日：2010-04-27

    申请人： Mehdi Khaled

    发明人： Mehdi Khaled

    IPC分类号： G01N27/02 ,  G01N27/00

    CPC分类号： A61B5/14532 ,  A61B5/1486 ,  B01L3/502715 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/024 ,  B01L2300/0816 ,  B01L2400/0478 ,  Y10T436/14 ,  Y10T436/142222 ,  Y10T436/144444

    摘要： The application provides a laboratory. The laboratory (400) comprises a portable casing (404). The portable casing (404) comprises a tray unit (407), an actuator unit (405, 412), an analyzer unit (419), and a communication unit (402, 415, 438).The tray unit (407) is used for receiving a cartridge (409). The cartridge (409) comprises an analyte reservoir (430) for receiving an analyte fluid, one or more chemical reagent reservoirs (432) for storing one or more chemical reagent fluids, and one or more channels (434) connecting the chemical reagent reservoirs (432) with the analyte reservoir (430). The channel (434) comprises a measurement area (436) while the measure measurement area (436) comprises a sensor. The actuator unit (405, 412) is used for reducing the volume of the analyte reservoir (430) and for reducing the volume of the chemical reagent reservoirs (432). The analyzer unit (419) is used for measuring a physical value in the measurement area (436) using the sensor. The communication unit (402, 415, 438) is used for outputting the physical value.

    摘要翻译： 该应用程序提供了一个实验室。 实验室（400）包括便携式外壳（404）。 便携式外壳（404）包括托盘单元（407），致动器单元（405,412），分析器单元（419）和通信单元（402,415,438）。 托盘单元（407）用于容纳盒（409）。 墨盒（409）包括用于接收分析物流体的分析物储存器（430），用于存储一种或多种化学试剂流体的一个或多个化学试剂储存器（432）和连接化学试剂储存器的一个或多个通道（434） 432）与分析物储存器（430）。 通道（434）包括测量区域（436），而测量区域（436）包括传感器。 致动器单元（405,412）用于减小分析物储存器（430）的体积并且用于减小化学试剂储存器（432）的体积。 分析器单元（419）用于使用传感器来测量测量区域（436）中的物理值。 通信单元（402,415,438）用于输出物理值。