公开(公告)号：US11202797B2

公开(公告)日：2021-12-21

申请号：US16762147

申请日：2018-07-11

Applicant: ZHEJIANG UNIVERSITY

Inventor： Shiguo Chen ,  Lufeng Yan ,  Danli Wang ,  Junhui Li ,  Xingqian Ye ,  Donghong Liu

IPC: A61K31/737 ,  B01D61/14 ,  C08B37/00 ,  A61P7/02 ,  A61P9/10

Abstract: The present invention discloses a mixture of fucosylated chondroitin sulfate oligosaccharides having an average molecular weight between 2700 Da and 3600 Da, in which 90% of total components has a molecular weight between 1800 Da and 5400 Da. The mixture of fucosylated chondroitin sulfate oligosaccharide of the present invention has very low anti-FIIa factor activity, very low anti-FXa factor activity, very high anti-FXase factor activity, i.e., having a significant activity in selectively inhibiting intrinsic FXase, and a significant antithrombotic activity and no risk of bleeding and hypotension that are proved through animal experiments. The present invention also discloses a method for rapidly producing fucosylated chondroitin sulfate oligosaccharides by using sea cucumber as raw material, and the method includes: intercepting and concentrating crude polysaccharide with a membrane, deacetylation with hydrazine, pyrolysis with nitrous acid, reduction with sodium borohydride, membrane separation, etc., achieving rapid preparation and high yield.