公开(公告)号：US08597950B2

公开(公告)日：2013-12-03

申请号：US10586998

申请日：2005-01-19

申请人： Sylvestre Marillonnet ,  Carola Engler ,  Victor Klimyuk ,  Yuri Gleba

发明人： Sylvestre Marillonnet ,  Carola Engler ,  Victor Klimyuk ,  Yuri Gleba

IPC分类号： C12N15/86 ,  A61K38/00 ,  C12N7/00 ,  C07K14/415 ,  C07K14/005 ,  C07H21/04 ,  C12N5/04

CPC分类号： C12N15/8216

摘要： A process for replicating or for replicating and expressing a sequence of interest in a plant, comprising: (i) an RNA replicon or a precursor thereof, said RNA replicon being derived from a plus-sense single stranded RNA virus and comprising at least one sequence of interest; and (ii) a helper replicon, or a precursor thereof, wherein said helper replicon is (a) incapable of systemic movement in said plant both in the presence and in the absence of said RNA replicon (i) and (b) capable of expressing in a plant one or more proteins necessary for systemic movement of said RNA replicon (i), whereby said RNA replicon (i) is capable of replicating or replicating and expressing said sequence of interest in said plant, but unable to move systemically in said plant in the absence of said one or more proteins expressed by said helper replicon (ii).

摘要翻译： 一种在植物中复制或表达感兴趣的序列的方法，包括：（i）RNA复制子或其前体，所述RNA复制子衍生自正义单链RNA病毒，并且包含至少一个序列 出于兴趣; 辅助复制子或其前体，其中所述辅助复制子是（a）在所述RNA复制子（i）和（b）能够表达的存在和不存在下，在所述植物中不能全身运动 在植物中，所述R​​NA复制子（i）的系统运动所必需的一种或多种蛋白质，其中所述RNA复制子（i）能够在所述植物中复制或复制和表达所述感兴趣的序列，但不能在所述植物中系统地移动 在不存在由所述辅助复制子（ii）表达的所述一种或多种蛋白质的情况下。

公开(公告)号：US20070044170A1

公开(公告)日：2007-02-22

申请号：US10578962

申请日：2004-11-10

申请人： Sylvestre Marillonnet ,  Carola Engler ,  Victor Kimyuk ,  Yuri Gleba

发明人： Sylvestre Marillonnet ,  Carola Engler ,  Victor Kimyuk ,  Yuri Gleba

IPC分类号： A01H1/00 ,  C12N15/82 ,  C12Q1/68

CPC分类号： C12N15/8203 ,  C12N15/8216

摘要： A process of expressing a sequence of interest in a plant, plant part, or plant cell culture, comprising: (a) providing a plant, plant part, or plant cell culture containing in cell nuclei a heterologous DNA having a sequence encoding an RNA replicon operably linked or linkable to a transcription promoter, wherein said sequence encoding an RNA replicon contains (i) sequences for replicon function of said RNA replicon, said sequences being derived from a sequence of a plant RNA virus, (ii) a sequence of interest, whereby said sequences for replicon function exhibit at selected localities of said sequences of said plant RNA virus functionconservative differences from said sequence of said plant RNA virus, said differences causing an increased frequency of replicon formation compared to an RNA replicon not exhibiting said differences; and (b) causing expression of said sequence of interest.

摘要翻译： 在植物，植物部分或植物细胞培养物中表达感兴趣的序列的方法，包括：（a）提供在细胞核中含有编码RNA复制子的序列的异源DNA的植物，植物部分或植物细胞培养物 其可编码RNA复制子的所述序列包含（i）所述RNA复制子的复制子功能的序列，所述序列衍生自植物RNA病毒的序列，（ii）感兴趣的序列， 其中所述用于复制子功能的序列在所述植物RNA病毒功能的所述序列的所选序列的所选位点处表现出与所述植物RNA病毒的所述序列相比的所述差异，所述差异导致复制子形成的频率增加，与不显示所述差异的RNA复制子相比; 和（b）引起所述感兴趣序列的表达。

公开(公告)号：US20070016983A1

公开(公告)日：2007-01-18

申请号：US10581703

申请日：2004-12-03

申请人： Stefan Muhlbauer

发明人： Stefan Muhlbauer

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8214 ,  C12N15/8238

摘要： A process of controlling expression of a plastome-encoded sequence of interest in a plant or in plant cells by externally applying to said plant or to said plant cells a chemical or physical control signal, wherein said control signal is adapted for interacting with an intra-plastid component of the plastid protein expression machinery and wherein expression of said sequence of interest is controlled by said control signal.

摘要翻译： 通过向所述植物或所述植物细胞外部施用化学或物理控制信号来控制植物或植物细胞中感兴趣的质体组编码序列的表达的方法，其中所述控制信号适于与植物细胞内相互作用， 质体蛋白质表达机制的质体组分，并且其中所述感兴趣序列的表达由所述控制信号控制。