公开(公告)号：US20200371087A1

公开(公告)日：2020-11-26

申请号：US16635483

申请日：2018-07-31

Applicant: Benaroya Research Institute at Virginia Mason

Inventor： Eric Wambre ,  William Kwok

IPC: G01N33/50 ,  C12N5/0783 ,  C07K14/73 ,  C07K14/705 ,  C07K14/54

Abstract: The disclosure provides methods and compositions for labelling, detecting, quantifying, and monitoring activated allergen-specific T cells. The disclosed methods and compositions can be applied to further detect allergic states or monitor therapies for allergies in subjects in need thereof.

公开(公告)号：US10363299B2

公开(公告)日：2019-07-30

申请号：US15551315

申请日：2016-02-19

Applicant: University of Virginia Patent Foundation ,  Benaroya Research Institute at Virginia Mason

Inventor： Judith Ann Woodfolk ,  Lyndsey M. Muehling ,  William W. Kwok ,  Duy Tran Mai

IPC: A61K38/00 ,  C07K14/005 ,  C07K14/02 ,  A61K39/00 ,  C07K14/155 ,  A61K39/12 ,  C12Q1/70 ,  G01N33/569

Abstract: An analysis of human CD4+ T-cell epitopes of RV capsid proteins with cross-reactive potential was performed, peptide epitopes of RV-A16 capsid proteins VP1 and VP2 were identified, RV-specific CD4+ T cells were phenotyped for surface markers and cytokine profiles using flow cytometry, and it was found that, among non-infected subjects, circulating RV-A16-specific CD4+ T cells detected at the highest frequencies targeted 10 unique epitopes with diverse HLA-DR binding capacity. T-cell epitopes localized to conserved regions of significance to the virus and were enriched for HLA class I and II binding motifs and were activated in vivo after experimental infection with RV-A16. RV-A16 epitopes constituted species-specific and pan-species varieties, together providing ˜90% coverage of the US population. Cross-reactivity was evidenced for RV-A16 and RV-A39. High-frequency circulating RV-specific memory Th1 cells in healthy individuals preferentially target a limited set of conserved epitopes and these epitopes, separately or combined, can serve as vaccines.