公开(公告)号：US5156968A

公开(公告)日：1992-10-20

申请号：US210909

申请日：1988-06-24

Applicant: Chung-Cheng Liu

Inventor： Chung-Cheng Liu

IPC: C07K14/00 ,  C07K14/64 ,  C12N9/60 ,  C12N15/57

CPC classification number: C12N9/60 ,  C07K14/00 ,  C07K14/64 ,  C07K2319/00 ,  C07K2319/036 ,  C07K2319/75 ,  C07K2319/95

Abstract: Ubiquitin hydrolase is provided having a purity of at least 70% homogeneity based on the weight of the total protein in the composition, which hydrolase hydrolyzes a ubiquitin-polypeptide conjugate at the amide bond linking the ubiquitin and polypeptide, thereby yielding intact polypeptide with an unconjugated, mature N-terminus. Also provided are DNA sequences encoding such ubiquitin hydrolase, as well as expression systems for its recombinant production. Processes are provided for purification of ubiquitin hydrolase from eukaryotes and for its use in recovering any desired polypeptide free from its fusion at its N-terminus with ubiquitin.

Abstract translation: 提供泛素水解酶，其纯度基于组合物中总蛋白质的重量至少70％的均匀性，该水解酶在连接泛素和多肽的酰胺键处水解泛素 - 多肽缀合物，由此产生具有未缀合的完整多肽 ，成熟N末端。 还提供了编码这种泛素水解酶的DNA序列，以及用于其重组生产的表达系统。 提供了用于从真核生物纯化泛素水解酶的方法，并且其用于在其N-末端用泛素回收任何无融合物的所需多肽。