公开(公告)号：US20110058164A1

公开(公告)日：2011-03-10

申请号：US12772118

申请日：2010-04-30

申请人： Xiang Zhang ,  Jonathan A. Ellman ,  Fanqing Frank Chen ,  Cheng Sun ,  Kai-Hang Su ,  Qi-Huo Wei

发明人： Xiang Zhang ,  Jonathan A. Ellman ,  Fanqing Frank Chen ,  Cheng Sun ,  Kai-Hang Su ,  Qi-Huo Wei

IPC分类号： G01J3/44

CPC分类号： G01N21/658 ,  B82Y15/00 ,  B82Y20/00 ,  C12Q1/37

摘要： A nanoplasmonic resonator (NPR) comprising a metallic nanodisk with alternating shielding layer(s), having a tagged biomolecule conjugated or tethered to the surface of the nanoplasmonic resonator for highly sensitive measurement of enzymatic activity. NPRs enhance Raman signals in a highly reproducible manner, enabling fast detection of protease and enzyme activity, such as Prostate Specific Antigen (paPSA), in real-time, at picomolar sensitivity levels. Experiments on extracellular fluid (ECF) from paPSA-positive cells demonstrate specific detection in a complex bio-fluid background in real-time single-step detection in very small sample volumes.

摘要翻译： 纳米等离子体共振器（NPR）包括具有交替屏蔽层的金属纳米盘，其具有缀合或连接到纳米塑料谐振器表面的标记生物分子，用于酶活性的高度灵敏测量。 NPR以高度可重复的方式增强拉曼信号，能够以皮摩尔敏感度水平实时快速检测蛋白酶和酶活性，如前列腺特异抗原（paPSA）。 来自paPSA阳性细胞的细胞外液（ECF）的实验在非常小的样品体积中在实时单步检测中在复杂的生物流体背景中表现出特异性检测。