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    Detection of protease and protease activity using a single nanoscrescent SERS probe
    1.
    发明授权
    Detection of protease and protease activity using a single nanoscrescent SERS probe 有权
    使用单个纳米SERS探针检测蛋白酶和蛋白酶活性

    公开(公告)号：US08361932B2

    公开(公告)日：2013-01-29

    申请号：US12299284

    申请日：2007-05-02

    申请人： Gang L. Liu ,  Jonathan A. Ellman ,  Luke P. Lee ,  Fanqing Frank Chen

    发明人： Gang L. Liu ,  Jonathan A. Ellman ,  Luke P. Lee ,  Fanqing Frank Chen

    IPC分类号： C40B30/04 ,  C40B40/10 ,  G01N33/573

    CPC分类号： C12Q1/37 ,  G01N33/57434 ,  G01N33/587 ,  G01N2333/96433 ,  G01N2333/96466 ,  G01N2333/974

    摘要： This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide.

    摘要翻译： 本发明涉及使用具有至少纳摩尔灵敏度的单个肽 - 缀合的纳米新月面增强拉曼散射（SERS）探针的体外检测蛋白酶。 该探针能够以非常小的体积和低浓度检测蛋白水解活性。 在某些实施方案中，探针包含用于检测活性蛋白酶的指示剂，其中指示剂包含连接到肽的纳米新月形，其中所述肽包含蛋白酶的识别位点和与肽连接的拉曼标签。

    SIZE-DEPENDENT BIOLOGICAL EFFECT OF NANOPARTICLES
    2.
    发明申请
    SIZE-DEPENDENT BIOLOGICAL EFFECT OF NANOPARTICLES 审中-公开
    纳米颗粒的大小依赖性生物学效应

    公开(公告)号：US20100279289A1

    公开(公告)日：2010-11-04

    申请号：US12601747

    申请日：2008-05-23

    申请人： Fanqing Frank Chen

    发明人： Fanqing Frank Chen

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6809 ,  B82Y5/00

    摘要： Nanoparticles are used increasingly in consumer products and biomedical applications. Yet the cellular interaction mechanism at the molecular level is not well understood for nanomaterials of different size, shape and surface chemistry. Gold nanoparticles (Au-NPs), which have been explored extensively for various applications in recent years, are used as the model system to help understand the size-dependent biological effects of nanoparticles. Jurkat cells treated with Au-NPs ranging from 2 nm to 200 nm were studied. Whole genome expression measurements indicate size-dependent effects, including linear scaling and threshold effects. In addition, a non-linear pattern of gene responses that persisted over time were observed in 20-40 nm Au-NP treated cells. Gene function, promoter, and pathway analyses reveal differential signaling processes that are correlated with nanoparticle sizes. The size may play a role in cellular sorting of naturally occurring particulates, particle interaction with the receptors, intracellular transportation, signaling and stress responses.

    摘要翻译： 纳米颗粒越来越多地用于消费品和生物医学应用。 然而，分子水平的细胞相互作用机制对于不同尺寸，形状和表面化学的纳米材料来说并不是很好理解。 近年来已经广泛应用于各种应用的金纳米颗粒（Au-NP）被用作模型系统，以帮助了解纳米颗粒的尺寸依赖性生物学效应。 研究了用Au-NP处理的Jurkat细胞，范围从2nm到200nm。 全基因组表达测量显示尺寸依赖性影响，包括线性缩放和阈值效应。 此外，在20-40nm Au-NP处理的细胞中观察到随时间持续的基因应答的非线性模式。 基因功能，启动子和通路分析揭示与纳米颗粒尺寸相关的差异信号传导过程。 大小可能在天然存在的颗粒的细胞分选，与受体的颗粒相互作用，细胞内运输，信号传导和应激反应中起作用。

    DETECTION OF PROTEASE AND PROTEASE ACTIVITY USING A SINGLE NANOSCRESCENT SERS PROBE
    4.
    发明申请
    DETECTION OF PROTEASE AND PROTEASE ACTIVITY USING A SINGLE NANOSCRESCENT SERS PROBE 有权
    使用单个纳米粒子探针检测蛋白质和蛋白酶活性

    公开(公告)号：US20100048412A1

    公开(公告)日：2010-02-25

    申请号：US12299284

    申请日：2007-05-02

    申请人： Gang L. Liu ,  Jonathan A. Ellman ,  Luke P. Lee ,  Fanqing Frank Chen

    发明人： Gang L. Liu ,  Jonathan A. Ellman ,  Luke P. Lee ,  Fanqing Frank Chen

    IPC分类号： C40B30/04 ,  G01N33/573 ,  C40B40/10

    CPC分类号： C12Q1/37 ,  G01N33/57434 ,  G01N33/587 ,  G01N2333/96433 ,  G01N2333/96466 ,  G01N2333/974

    摘要： This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide.

    摘要翻译： 本发明涉及使用具有至少纳摩尔灵敏度的单个肽 - 缀合的纳米新月面增强拉曼散射（SERS）探针的体外检测蛋白酶。 该探针能够以非常小的体积和低浓度检测蛋白水解活性。 在某些实施方案中，探针包含用于检测活性蛋白酶的指示剂，其中指示剂包含连接到肽的纳米新月形，其中所述肽包含蛋白酶的识别位点和与肽连接的拉曼标签。

    Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity
    5.
    发明授权
    Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity 有权
    使用具有超高灵敏度的纳米级谐振器进行实时，单步生物测定

    公开(公告)号：US08685743B2

    公开(公告)日：2014-04-01

    申请号：US12772118

    申请日：2010-04-30

    申请人： Xiang Zhang ,  Jonathan A. Ellman ,  Fanqing Frank Chen ,  Kai-Hang Su ,  Qi-Huo Wei ,  Cheng Sun

    发明人： Xiang Zhang ,  Jonathan A. Ellman ,  Fanqing Frank Chen ,  Kai-Hang Su ,  Qi-Huo Wei ,  Cheng Sun

    IPC分类号： G01N21/00

    CPC分类号： G01N21/658 ,  B82Y15/00 ,  B82Y20/00 ,  C12Q1/37

    摘要： A nanoplasmonic resonator (NPR) comprising a metallic nanodisk with alternating shielding layer(s), having a tagged biomolecule conjugated or tethered to the surface of the nanoplasmonic resonator for highly sensitive measurement of enzymatic activity. NPRs enhance Raman signals in a highly reproducible manner, enabling fast detection of protease and enzyme activity, such as Prostate Specific Antigen (paPSA), in real-time, at picomolar sensitivity levels. Experiments on extracellular fluid (ECF) from paPSA-positive cells demonstrate specific detection in a complex bio-fluid background in real-time single-step detection in very small sample volumes.

    摘要翻译： 纳米等离子体共振器（NPR）包括具有交替屏蔽层的金属纳米盘，其具有缀合或连接到纳米塑料谐振器表面的标记生物分子，用于酶活性的高度灵敏测量。 NPR以高度可重复的方式增强拉曼信号，能够以皮摩尔敏感度水平实时快速检测蛋白酶和酶活性，如前列腺特异抗原（paPSA）。 来自paPSA阳性细胞的细胞外液（ECF）的实验在非常小的样品体积中在实时单步检测中在复杂的生物流体背景中表现出特异性检测。

    Preassembled hybrid nanocluster plasmonic resonator for immunological detection and serotyping of virus and microbes
    7.
    发明申请
    Preassembled hybrid nanocluster plasmonic resonator for immunological detection and serotyping of virus and microbes 审中-公开
    用于免疫检测和病毒和微生物血清分型的预组装的混合纳米团簇等离子体振子

    公开(公告)号：US20150065694A1

    公开(公告)日：2015-03-05

    申请号：US14472516

    申请日：2014-08-29

    申请人： Fanqing Frank Chen ,  Mohamed Shehata Draz

    发明人： Fanqing Frank Chen ,  Mohamed Shehata Draz

    IPC分类号： G01N33/569 ,  C07K17/14

    CPC分类号： C07K17/14 ,  B82Y15/00 ,  G01N33/5761 ,  G01N33/5767 ,  G01N33/588 ,  G01N2333/05 ,  G01N2458/15 ,  G01N2469/10 ,  Y10S977/774 ,  Y10S977/81

    摘要： Here, we describe a preassembled plasmonic resonance nanocluster. One embodiment is used for microbe detection and typing. The metallic nanoparticle acceptors with microbe surface antigen epitope, and quantum dot (QD) donors with Fab antibody, are assembled into an immuno-mediated 3D-oriented complex with enhanced energy transfer and fluorescence quenching. The coherent plasmonic resonance between the metal and QD nanoparticles is exploited to achieve improved donor-acceptor resonance within the nanocluster, which in the presence of microbial particles is disassembled in a highly specific manner. The nanocluster provides high detection specificity and sensitivity of the microbes, with a sensitivity limit down to 1-100 particles per microliter and to attomolar levels of a surface antigen epitope. A few specific examples of the plasmonic resonance nanocluster used in microbe detection are disclosed along with ways in which the complex can be easily modified for additional microbes.

    摘要翻译： 在这里，我们描述一个预装配的等离子体共振纳米簇。 一个实施例用于微生物检测和分型。 具有微生物表面抗原表位的金属纳米颗粒受体和具有Fab抗体的量子点（QD）供体被组装成具有增强的能量转移和荧光猝灭的免疫介导的3D取向复合物。 利用金属和QD纳米颗粒之间的相干等离子体共振来实现在纳米簇内改善的供体 - 受体共振，其在微生物颗粒存在下以高度特异性的方式被分解。 纳米团簇提供了微生物的高检测特异性和敏感性，灵敏度限制为每微升1-100个颗粒和表面抗原表位的水摩尔浓度。 公开了微生物检测中使用的等离子体共振纳米簇的一些具体实例，以及复合物可以容易地为其它微生物修饰的方式。

    Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting
    8.
    发明授权
    Nanoplasmonic molecular ruler for nuclease activity and DNA footprinting 有权
    用于核酸酶活性和DNA足迹的纳米质谱分子尺

    公开(公告)号：US08569468B2

    公开(公告)日：2013-10-29

    申请号：US12440579

    申请日：2007-09-14

    申请人： Fanqing Frank Chen ,  Gang L. Liu ,  Luke P. Lee

    发明人： Fanqing Frank Chen ,  Gang L. Liu ,  Luke P. Lee

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/683 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6876 ,  G01N21/554 ,  C12Q2565/628 ,  C12Q2563/155 ,  C12Q2521/319

    摘要： This invention provides a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of nucleic acid (e.g., DNA) length changes and perform nucleic acid footprinting. In various embodiments the ruler comprises a nucleic acid attached to a nanoparticle, such that changes in the nucleic acid length are detectable using surface plasmon resonance. The nanoplasmonic ruler provides a fast and convenient platform for mapping nucleic acid-protein interactions, for nuclease activity monitoring, and for other footprinting related methods.

    摘要翻译： 本发明提供了一种纳米质谱分子尺，其可进行核酸（例如DNA）长度变化的无标记和实时监测并进行核酸足迹。 在各种实施方案中，尺子包括连接到纳米颗粒的核酸，使得可以使用表面等离子体共振来检测核酸长度的变化。 纳米尺度标尺为核酸 - 蛋白质相互作用，核酸酶活性监测和其他足迹相关方法提供了一个快捷方便的平台。

    NANOPLASMONIC MOLECULAR RULER FOR NUCLEASE ACTIVITY AND DNA FOOTPRINTING
    9.
    发明申请
    NANOPLASMONIC MOLECULAR RULER FOR NUCLEASE ACTIVITY AND DNA FOOTPRINTING 有权
    用于核酸活性的NANOPLASMONIC分子规则和DNA加工

    公开(公告)号：US20100323906A1

    公开(公告)日：2010-12-23

    申请号：US12440579

    申请日：2007-09-14

    申请人： Fanqing Frank Chen ,  Gang L. Liu ,  Luke P. Lee

    发明人： Fanqing Frank Chen ,  Gang L. Liu ,  Luke P. Lee

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C40B40/08 ,  C40B30/04 ,  C07H21/04

    CPC分类号： C12Q1/683 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6876 ,  G01N21/554 ,  C12Q2565/628 ,  C12Q2563/155 ,  C12Q2521/319

    摘要： This invention provides a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of nucleic acid (e.g., DNA) length changes and perform nucleic acid footprinting. In various embodiments the ruler comprises a nucleic acid attached to a nanoparticle, such that changes in the nucleic acid length are detectable using surface plasmon resonance. The nanoplamonic ruler provides a fast and convenient platform for mapping nucleic acid -protein interactions, for nuclease activity monitoring, and for other footprinting related methods.

    摘要翻译： 本发明提供了一种纳米质谱分子尺，其可进行核酸（例如DNA）长度变化的无标记和实时监测并进行核酸足迹。 在各种实施方案中，尺子包括连接到纳米颗粒的核酸，使得可以使用表面等离子体共振来检测核酸长度的变化。 纳米尺度标尺为核酸 - 蛋白质相互作用，核酸酶活性监测和其他足迹相关方法提供了一个快捷方便的平台。