公开(公告)号：US6096563A

公开(公告)日：2000-08-01

申请号：US999739

申请日：1997-09-30

申请人： Kiamars Hajizadeh ,  Robert T. Hudak ,  James W. Stave

发明人： Kiamars Hajizadeh ,  Robert T. Hudak ,  James W. Stave

IPC分类号： G01N33/543 ,  G01N33/533

CPC分类号： G01N33/54313 ,  Y10S436/808 ,  Y10S436/81 ,  Y10S436/824

摘要： A dual particle immunoassay method and kit for detecting analyte in a sample in which the sample to be analyzed, a binding molecule specific for the analyte, and a particle coated with the analyte to be detected or coated with a second binding molecule are reacted and applied to a porous membrane. The competitive immunoassay utilizes an analyte-coated particle, whereas the sandwich immunoassay employs a second binding molecule-coated particle. All of the reagents except for the coated particle are able to pass through the porous membrane. Detectable particles coated with a binding substance that binds to the binding molecule, such as protein A protein G, second antibody reactive to the binding molecule, or a small synthetic affinity ligand, are reacted with coated particles retained on the membrane surface. The detectable particles will pass through the membrane if not complexed with the coated particle. In the competitive immunoassay, detectable particles bind to binding molecules that complex with the analyte-coated particles in the absence of analyte and are detected. In the sandwich immunoassay, detectable particles bind to binding molecules that are attached to the coated particle in the presence of analyte and are detected.

摘要翻译： 用于检测样品中分析物的双重颗粒免疫测定方法和试剂盒，其中待分析的样品，分析物特异性的结合分子和用待检测或用第二结合分子包被的分析物的颗粒进行反应和应用 到多孔膜。 竞争性免疫测定使用分析物包被的颗粒，而夹心免疫测定采用第二结合分子包被的颗粒。 除了涂覆颗粒之外的所有试剂都能够通过多孔膜。 与结合分子结合的可结合物质如蛋白质A蛋白G，与结合分子反应的第二抗体或小的合成亲和配体包被的可检测颗粒与保留在膜表面上的被覆颗粒反应。 如果不与涂覆的颗粒络合，可检测的颗粒将通过膜。 在竞争性免疫测定中，可检测的颗粒结合到在不存在分析物的情况下与分析物包被的颗粒复合的结合分子并被检测。 在夹心免疫测定中，可检测的颗粒与分析物存在下连接到包被颗粒上的结合分子结合并被检测。

公开(公告)号：US5994145A

公开(公告)日：1999-11-30

申请号：US652643

申请日：1996-05-31

申请人： James W. Stave ,  Cynthia A. Kozakiewicz ,  Dale V. Onisk ,  Robert T. Hudak

发明人： James W. Stave ,  Cynthia A. Kozakiewicz ,  Dale V. Onisk ,  Robert T. Hudak

IPC分类号： G01N33/53 ,  G01N33/531 ,  G01N33/00 ,  G01N33/566

CPC分类号： G01N33/5308 ,  G01N33/531 ,  Y10T436/21 ,  Y10T436/216 ,  Y10T436/23

摘要： Reagents, immunoassay methods, and kits for detecting the presence or amount of trichloroethylene or perchloroethylene in a sample. Reagents include antibodies, immunogens for preparing antibodies, and assay conjugates. The immunogens elicit high affinity antibodies to trichloroethylene. The antibodies have minimal crossreactivity to other halogenated and aromatic hydrocarbons. The assay conjugates provide for sufficient binding of the antibody to the conjugate with maximum inhibition of that binding by free trichloroethylene and perchloroethylene in the sample. The reagents are useful in immunoassays to detect trichloroethylene and perchloroethylene, especially in environmental samples. Kits contain antibodies specific for trichloroethylene and perchloroethylene and assay conjugates.

摘要翻译： 用于检测样品中三氯乙烯或全氯乙烯的存在或量的试剂，免疫测定方法和试剂盒。 试剂包括抗体，用于制备抗体的免疫原和测定缀合物。 免疫原引起三氯乙烯的高亲和力抗体。 该抗体与其它卤代和芳香烃具有最小的交叉反应性。 测定缀合物提供抗体与缀合物的充分结合，同时最大限度地抑制样品中游离三氯乙烯和全氯乙烯的结合。 这些试剂可用于免疫测定以检测三氯乙烯和全氯乙烯，特别是在环境样品中。 试剂盒含有对三氯乙烯和全氯乙烯和测定缀合物特异的抗体。