公开(公告)号：US20190112656A1

公开(公告)日：2019-04-18

申请号：US16088596

申请日：2017-03-25

申请人： KAWASAKI GAKUEN EDUCATIONAL FOUNDATION

发明人： Yoshihide SUNADA ,  Yutaka OHSAWA ,  Shin-ichiro NISHIMATSU

IPC分类号： C12Q1/6876 ,  A61Q19/08 ,  A23L33/18 ,  A61K31/185 ,  A61K8/46 ,  C12Q1/6809 ,  C12Q1/6825

摘要： The present invention provides a method for measuring senescence of a test subject, including a step of measuring a level of taurine modification of mitochondrial tRNALeu(UUR) in a biological sample isolated from a test subject, by a reverse transcription reaction from a primer with the tRNALeu(UUR) as a template. In addition, the present invention also provides a senescence determining drug containing a primer containing a base sequence consisting of 10-25 bases and complementary to the mitochondrial tRNALeu(UUR). Furthermore, the present invention also provides a senescence determining kit containing a primer containing a base sequence consisting of 10-25 bases and complementary to the mitochondrial tRNALeu(UUR) and a reverse transcriptase. Moreover, the present invention also provides an agent for improving a taurine modification rate of mitochondrial tRNALeu(UUR), containing taurine as an active ingredient.

公开(公告)号：US20180251760A1

公开(公告)日：2018-09-06

申请号：US15759305

申请日：2016-09-16

申请人： NIPPON SHINYAKU CO., LTD. ,  Kawasaki Gakuen Educational Foundation

发明人： Shinichiro NAKAGAWA ,  Seigo NAGATA ,  Yoshihide SUNADA ,  Yutaka OHSAWA ,  Shin-ichiro NISHIMATSU

IPC分类号： C12N15/113

CPC分类号： C12N15/113 ,  A61K31/7088 ,  C12N15/1136 ,  C12N2310/11 ,  C12N2310/31 ,  C12N2310/32 ,  C12N2310/3233 ,  C12N2310/3519 ,  C12N2320/33

摘要： There has been a demand for a novel antisense nucleic acid or the like capable of inhibiting myostatin at the mRNA level.The present invention provides a specific antisense oligomer which allows exon 2 skipping in the myostatin gene or induces degradation of mRNA of the myostatin gene.