公开(公告)号：US20130288266A1

公开(公告)日：2013-10-31

申请号：US13923618

申请日：2013-06-21

申请人： Michael Gerg ,  Dieter Heindl ,  Christian Klein ,  Alfred Mertens ,  Volker Schmid ,  Michael Schraeml ,  Monika Soukupova ,  Michael Tacke

发明人： Michael Gerg ,  Dieter Heindl ,  Christian Klein ,  Alfred Mertens ,  Volker Schmid ,  Michael Schraeml ,  Monika Soukupova ,  Michael Tacke

IPC分类号： C07K16/46

CPC分类号： C07K16/468 ,  G01N33/531

摘要： A bi-valent binding agent having a first monovalent binder that binds to a polypeptide epitope of a target polypeptide, a second monovalent binder that binds to a posttranslational polypeptide modification on the target polypeptide and a linker. Further disclosed are methods for the detection of a posttranslationally modified target polypeptide, for making the disclosed bi-valent binding agent, and for use of the disclosed bi-valent binding agent in histological staining procedures.

摘要翻译： 具有结合靶多肽的多肽表位的第一单价结合剂的双价结合剂，与靶多肽上的翻译后多肽修饰结合的第二单价粘合剂和接头。 进一步公开的是用于检测翻译后修饰的靶多肽，用于制备所公开的双价结合剂的方法，以及用于所公开的双价结合剂在组织学染色程序中的方法。

公开(公告)号：US20110160436A1

公开(公告)日：2011-06-30

申请号：US13060535

申请日：2009-08-25

申请人： Michael Schraeml ,  Leopold Von Proff

发明人： Michael Schraeml ,  Leopold Von Proff

IPC分类号： C07K16/00 ,  G01N33/557 ,  G01N33/53

CPC分类号： G01N33/6854 ,  C07K16/26 ,  G01N21/553

摘要： The current invention reports a method for producing an antibody comprising the steps of a) providing a plurality of hybridoma cells each expressing an antibody, b) determining the time dependent amount of said antibody bound to the respective antigen by surface plasmon resonance at different temperatures and different antibody concentrations, c) calculating with the time dependent amount determined in b) based on equations (II) to (XIII) at least the thermodynamic parameters (i) standard association binding entropy formula (A), (ii) standard dissociation binding entropy formula (B), (iii) standard binding entropy (ΔS°), (iv) free standard binding enthalpy (ΔG°), (v) standard dissociation free binding enthalpy formula (C), (vi) standard association free binding enthalpy formula (D), (vii) −TΔS°, (viii) dissociation rate constant kd, (ix) equilibrium binding constant KD, and (x) association rate constant ka, d) selecting a hybridoma cell producing an antibody with at least two of the following: i) a standard association binding entropy of less than 10 J/K*mol, ii) an absolute standard dissociation binding entropy of 100 J/mol*K or more, iii) an absolute standard binding entropy of 100 J/mol*K or more, e) producing an antibody by cultivating said selected cell under conditions suitable for the expression of said antibody and recovering said antibody from the cells or/and the cultivation medium.

摘要翻译： 本发明报告了一种制备抗体的方法，包括以下步骤：a）提供多个表达抗体的杂交瘤细胞，b）通过在不同温度下的表面等离子体共振测定与相应抗原结合的所述抗体的时间依赖性量， 不同的抗体浓度，c）基于等式（II）至（XIII）至少在热力学参数（i）标准关联结合熵公式（A），（ii）标准解离结合熵 公式（B），（iii）标准结合熵（＆Dgr; S°），（iv）游离标准结合焓（＆Dgr; G°），（v）标准无离子结合焓式（C），（vi） 游离结合焓式（D），（vii）-T＆Dgr; S°，（viii）解离速率常数kd，（ix）平衡结合常数KD和（x）缔合速率常数ka，d）选择产生 抗体与le ast）以下两个：i）小于10J / K * mol的标准缔合结合熵，ii）100J / mol * K以上的绝对标准解离结合熵，iii）绝对标准结合熵100 J / mol * K或更多，e）通过在适于所述抗体表达的条件下培养所述选择的细胞产生抗体，并从细胞或培养基中回收所述抗体。

公开(公告)号：US20100130720A1

公开(公告)日：2010-05-27

申请号：US12514550

申请日：2007-11-15

申请人： Michael Schraeml ,  Erik Fiedler

发明人： Michael Schraeml ,  Erik Fiedler

IPC分类号： C07K14/00 ,  C12P21/06 ,  C07K1/00 ,  G01N33/53 ,  C40B50/00 ,  C12Q1/68 ,  C40B30/04 ,  C07H21/04

CPC分类号： C07K14/00 ,  G01N33/6803

摘要： The present invention is directed to a method for the generation of binding proteins derived from the protein super-family of ubiquitin like proteins with modifications in their alpha helical region as well as to a protein obtainable by said method. Furthermore, the invention provides the use of a protein for the specific recognition, binding and neutralization of a predescribed target molecule, for the detection, quantitative determination, separation and/or for the isolation of a corresponding binding partner and the use of a protein of the invention, for diagnosis, prophylaxis and treatment of diseases in which the corresponding binding partner is directly or indirectly involved.

摘要翻译： 本发明涉及一种用于产生衍生自泛蛋白样蛋白质的蛋白质超家族的结合蛋白质的方法，其修饰在其α螺旋区域以及可通过所述方法获得的蛋白质。 此外，本发明提供蛋白质用于预测的靶分子的特异性识别，结合和中和用于检测，定量测定，分离和/或分离相应结合配偶体的用途以及使用蛋白质 本发明用于诊断，预防和治疗其中相应的结合伴侣直接或间接参与的疾病。