公开(公告)号：US06500647B1

公开(公告)日：2002-12-31

申请号：US09463282

申请日：2000-01-20

申请人： Eun-Kyung Jung ,  Doo-Hong Park ,  Soo-Il Chung

发明人： Eun-Kyung Jung ,  Doo-Hong Park ,  Soo-Il Chung

IPC分类号： C12N1509

CPC分类号： C07K14/635 ,  C07K2319/02 ,  C07K2319/50 ,  C12N9/1205 ,  C12N15/70 ,  C12Y207/01019

摘要： The present invention relates to a recombinant expression vector which is prepared by inserting a human parathyroid hormone gene containing a urokinase-specific cleavage site into an L-arabinose inducible vector containing a phosphoribulokinase gene fragment of Rhodabacter sphaeroides or its mutated gene as a fusion partner, or its mutate gene as a fusion partner, a recombinant microorganism transformed with the said expression vector, and a process for preparing human parathyroid hormone on a large scale by cultivating the said microorganism in a medium containing L-arabinose. In accordance with the invention, a recombinant human PTH having the same activity of the native human PTH can be prepared in a high yield through the precise control of induction by a manufacturing process which comprises a step of inducing expression of fusion protein in the microorganism transformed with the recombinant expression vector by L-arabinose.

摘要翻译： 本发明涉及通过将含有尿激酶特异性切割位点的人甲状旁腺激素基因插入到含有红细菌杆菌的磷酸核糖激酶基因片段或其突变基因作为融合配偶体的L-阿拉伯糖诱导型载体中而制备的重组表达载体， 或其突变基因作为融合配偶体，用所述表达载体转化的重组微生物，以及通过在含有L-阿拉伯糖的培养基中培养所述微生物大规模制备人甲状旁腺激素的方法。 根据本发明，具有相同活性的天然人PTH的重组人PTH可以通过制备方法的精确控制诱导，以高产率制备，其包括在转化的微生物中诱导融合蛋白的表达的步骤 与L-阿拉伯糖的重组表达载体。