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192 条记录 (耗时 0.026 秒)

    Cryptosporidium parvum antigens, antibodies thereto and diagnostic and therapeutic compositions thereof
    3.
    发明授权
    Cryptosporidium parvum antigens, antibodies thereto and diagnostic and therapeutic compositions thereof 失效
    隐孢子虫小球抗原，其抗体及其诊断和治疗组合物

    公开(公告)号：US06808900B2

    公开(公告)日：2004-10-26

    申请号：US09881556

    申请日：2001-06-14

    申请人： J. Neil Simonsen

    发明人： J. Neil Simonsen

    IPC分类号： C12N1509

    CPC分类号： C07K14/44 ,  A61K38/00 ,  A61K2039/505 ,  Y02A50/488 ,  Y10S435/975

    摘要： Cloning and expression of genes encoding C. parvum antigenic polypeptides are described as are antibodies that recognize epitopes on these polypeptides. The antigenic polypeptides and antibodies thereto can be used in therapeutic compositions for the prevention and treatment of C. parvum infections, as well as in diagnostic methods for determining the presence of C. parvum infections.

    摘要翻译： 编码小叶抗原多肽的基因的克隆和表达被描述为识别这些多肽上的表位的抗体。 抗原多肽及其抗体可用于预防和治疗小孢子虫感染的治疗组合物中，以及用于确定小球孢菌感染存在的诊断方法。

    Method of suppressing gene expression in plants
    5.
    发明授权
    Method of suppressing gene expression in plants 有权
    抑制植物基因表达的方法

    公开(公告)号：US06759571B1

    公开(公告)日：2004-07-06

    申请号：US09281528

    申请日：1999-03-30

    申请人： Dominique Robertson

    发明人： Dominique Robertson

    IPC分类号： C12N1509

    CPC分类号： C12N15/8201

    摘要： The introduction of DNA episomes into plant cells to reduce or prevent the expression of endogenous nuclear or chromosomal genes is described. Geminivirus vectors to provide systemic silencing of an endogenous plant gene in a treated plant are described.

    摘要翻译： 描述了将DNA附加体引入植物细胞以减少或预防内源性核或染色体基因的表达。 描述了在受处理的植物中提供内源植物基因的全身沉默的双子病毒载体。

    Compositions and methods for non-targeted activation of endogenous genes
    8.
    发明授权
    Compositions and methods for non-targeted activation of endogenous genes 有权
    内源基因非靶向激活的组成和方法

    公开(公告)号：US06670185B1

    公开(公告)日：2003-12-30

    申请号：US09479123

    申请日：2000-01-07

    申请人： John J. Harrington ,  Bruce Sherf ,  Stephen Rundlett

    发明人： John J. Harrington ,  Bruce Sherf ,  Stephen Rundlett

    IPC分类号： C12N1509

    CPC分类号： C07K14/705 ,  A61K48/00 ,  C07K2319/00 ,  C12N9/003 ,  C12N15/1096 ,  C12N15/63 ,  C12N15/67 ,  C12N15/85 ,  C12N15/90 ,  C12N2800/107 ,  C12N2800/108 ,  C12N2800/204 ,  C12N2800/60 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

    摘要： The present invention is directed generally to activating gene expression or causing over-expression of a gene by recombination methods in situ. The invention also is directed generally to methods for expressing an endogenous gene in a cell at levels higher than those normally found in the cell. In one embodiment of the invention, expression of an endogenous gene is activated or increased following integration into the cell, by non-homologous or illegitimate recombination, of a regulatory sequence that activates expression of the gene. The invention also provides methods for the identification, activation, isolation, and/or expression of genes undiscoverable by current methods since no target sequence is necessary for integration. The invention also provides methods for isolation of nucleic acid molecules (particularly cDNA molecules) encoding a variety of proteins. Thus, by the present invention, endogenous genes, including those associated with human disease and development, may be activated and isolated without prior knowledge of the sequence, structure, function, or expression profile of the genes.

    摘要翻译： 本发明一般涉及通过原位重组方法激活基因表达或引起基因过度表达。 本发明还通常涉及用于在细胞中以比通常在细胞中发现的那些更高的水平表达内源基因的方法。 在本发明的一个实施方案中，内源基因的表达在通过激活基因表达的调节序列的非同源或非法重组整合入细胞后被激活或增加。 本发明还提供了鉴定，激活，分离和/或表达不能被现有方法发现的基因的方法，因为不需要靶序列进行整合。 本发明还提供了分离编码多种蛋白质的核酸分子（特别是cDNA分子）的方法。 因此，通过本发明，可以在没有基因的序列，结构，功能或表达谱的事先知识的情况下激活和分离内源基因，包括与人类疾病和发育相关的内源基因。

    Trichothecene-resistant transgenic plants
    9.
    发明授权
    Trichothecene-resistant transgenic plants 有权
    抗逆转录病毒转基因植物

    公开(公告)号：US06646184B2

    公开(公告)日：2003-11-11

    申请号：US10074279

    申请日：2002-02-12

    申请人： Thomas M. Hohn ,  Cheryl Peters ,  John Salmeron

    发明人： Thomas M. Hohn ,  Cheryl Peters ,  John Salmeron

    IPC分类号： C12N1509

    CPC分类号： C07H21/04 ,  C12N9/1029 ,  C12N15/8209 ,  C12N15/8242 ,  C12N15/8274 ,  C12N15/8282

    摘要： The present invention discloses trichothecene-resistant transgenic plants, plant tissues, plant seeds, and plant cells comprising a heterologous polynucleotide encoding a gene product having tricothecene resistance activity that thereby confers trichothecene resistance to the transgenic plants, plant tissues, plant seeds, and plant cells. Trichothecene resistance activity, as used herein, refers to an activity that reduces or inhibits the phytotoxicity of a trichothecene, particularly to a fungus and/or plant. In a particular embodiment, trichothecene resistance activity refers to an activity that transfers an acetate to the C-3 position of a trichothecene such as T-2 toxin, HT-2 toxin, isotrichodermol, diacetoxyscirpenol (“DAS”), 3-deacetylcalonectrin, 3,15-dideacetylcalonectrin, scirpentriol, neosolaniol; 15-acetyldeoxynivalenol, nivalenol, 4-acetylnivalenol (fusarenone-X), 4,15-diacetylnivalenol, 4,7,15-acetylnivalenol, and deoxynivalenol (“DON”) and their various acetylated derivatives. In another particular embodiment, the gene product having trichthecene resistance activity is a 3-O-acetyltransferase from a trichothecene-producing species of Fusarium, such as Fusarium graminearum or Fusarium sporotrichioides.

    摘要翻译： 本发明公开了头孢霉烯抗性转基因植物，植物组织，植物种子和植物细胞，其包含编码具有三氯生物抗性活性的基因产物的异源多核苷酸，从而赋予转基因植物，植物组织，植物种子和植物细胞的单端孢霉烯抗性 。 如本文所用，三氯乙炔抗性活性是指减少或抑制单端孢霉烯特别是真菌和/或植物的植物毒性的活性。 在一个特定的实施方案中，单端孢霉烯抗性活性是指将乙酸酯转移到单端孢霉烯C-3位的活性，例如T-2毒素，HT-2毒素，异维甲酸，二乙酰氧基甲苯酚（“DAS”），3-脱乙酰基木聚糖， 3,15-二乙酰胆碱，顺丁三醇，新溶胶甘醇; 15-乙酰基过氧化雪腐镰刀菌烯醇，新戊烯醇，4-乙酰基雪腐镰刀菌烯醇（fisarenone-X），4,15-二乙酰基雪腐镰刀菌烯醇，4,7,15-乙酰基雪腐镰刀菌烯醇和脱氧雪腐镰刀菌烯醇（“DON”）及其各种乙酰化衍生物。 在另一个具体实施方案中，具有三辛抗性活性的基因产物是来自产生单端孢霉烯的镰刀菌属物种如禾谷镰刀菌（Fusarium graminearum）或孢子镰刀菌（Fusarium sporotrichioides）的3-O-乙酰转移酶。