公开(公告)号：US10066019B2

公开(公告)日：2018-09-04

申请号：US14435094

申请日：2013-08-21

Applicant: Agency for Science, Technology and Research

Inventor： Zhiwei Song

IPC: C07K16/22 ,  C07K16/32 ,  C07K16/28 ,  C12N15/63 ,  C07K16/24 ,  C07K16/30

Abstract: Antibodies of interest comprising a heavy chain signal peptide and/or a light chain signal peptide or combination thereof and compositions are described. The method of determining the signal peptide including creating data set of antibodies signal peptides, clustering, selecting and creating recombinant antibodies for enhanced expression and secretion are described.

公开(公告)号：US20150152402A1

公开(公告)日：2015-06-04

申请号：US14620856

申请日：2015-02-12

Applicant: Agency for Science, Technology and Research

Inventor： Zhiwei Song

IPC: C12N9/42 ,  C12P21/00

CPC classification number: C12N9/2445 ,  C12N9/2402 ,  C12P21/005 ,  C12Y204/01101 ,  C12Y302/01045

Abstract: We describe a method of expressing a recombinant protein comprising mannose-terminated N-glycans from a host cell, the method comprising: (a) introducing a nucleic acid encoding a recombinant protein into a Chinese Hamster Ovary (CHO) cell comprising a mutation in the GnT 1 gene (GenBank Accession Number AF343963) leading to loss of GnT 1 function; and (c) expressing the recombinant protein from the host cell, in which the expressed recombinant protein comprises a mannose-terminated glycan structure, and in which the method does not include a step of introducing functional GnT-I into the host cell. The method may be used for producing recombinant glucocerebrosidase with a mannose-terminated glycan structure, suitable for treatment or prevention of Gaucher's Disease.

Abstract translation: 我们描述了从宿主细胞中表达包含甘露糖封端的N-聚糖的重组蛋白的方法，所述方法包括：（a）将编码重组蛋白的核酸引入中国仓鼠卵巢（CHO）细胞中，所述中国仓鼠卵巢（CHO）细胞包含 GnT 1基因（GenBank登录号AF343963）导致GnT1功能丧失; 和（c）从宿主细胞中表达重组蛋白，其中表达的重组蛋白包含甘露糖封端的聚糖结构，并且其中该方法不包括将功能性GnT-I引入宿主细胞的步骤。 该方法可用于生产具有甘露糖封端的聚糖结构的重组葡糖脑苷脂酶，适用于治疗或预防戈谢氏病。