公开(公告)号：US20050050581A1

公开(公告)日：2005-03-03

申请号：US10496731

申请日：2002-12-02

申请人： Alex Harvey ,  Youliang Wang

发明人： Alex Harvey ,  Youliang Wang

IPC分类号： A01K67/027 ,  A01K67/00 ,  C07K14/56 ,  C07K14/81 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  C12N15/09 ,  C12N15/11 ,  C12N15/85 ,  C07H21/04 ,  C07K14/47 ,  C12N5/06

CPC分类号： C07K14/8135 ,  A01K2227/30 ,  C07K14/56 ,  C12N2799/021

摘要： The present invention provides novel isolated nucleic acids that comprise an avian nucleic acid sequence comprising a ovomucoid gene expression control region. The ovomucoid promoter region of the present invention will allow expression of an operably linked heterologous nucleic acid insert in a transfected avian cell such as, for example, an oviduct cell. The isolated avian ovomucoid of the present invention may be operably linked with a selected nucleic acid insert, wherein the nucleic acid insert encodes a polypeptide desired to be expressed in a transfected cell. The recombinant DNA of the present invention may further comprise a polyadenylation signal sequence. The present invention further includes expression vectors comprising an isolated avian ovomucoid gene expression control region of the present invention, and transfected cells and transgenic avians comprising the expression vectors.

摘要翻译： 本发明提供新的分离的核酸，其包含包含卵巢粘蛋白基因表达控制区的禽类核酸序列。 本发明的卵巢粘蛋白启动子区域将允许在经转染的禽类细胞例如输卵管细胞中表达可操作地连接的异源核酸插入物。 本发明的分离的禽类卵类粘蛋白可以与所选择的核酸插入物可操作地连接，其中核酸插入物编码期望在转染细胞中表达的多肽。 本发明的重组DNA还可以包含聚腺苷酸化信号序列。 本发明还包括包含本发明的分离的禽类卵巢粘蛋白基因表达控制区的表达载体，以及包含表达载体的转染细胞和转基因鸟类。