公开(公告)号：US20090204337A1

公开(公告)日：2009-08-13

申请号：US12029393

申请日：2008-02-11

Applicant: Amol Prakash

Inventor： Amol Prakash

IPC: G01N33/00 ,  G06F19/00

CPC classification number: G01N30/8693 ,  G01N30/8644 ,  G01N30/8675 ,  G01N33/6803 ,  G01N33/6848

Abstract: A method for determining a time of elution of a peptide of interest from a liquid chromatography column includes a step of obtaining chromatographic data for each of a plurality of candidate fragment ions of the peptide of interest. A time along a common chromatographic time is scale determined corresponding to maximum overlay of the ion signals measured for each of the plurality of candidate fragment ions. Finally, the determined time is assigned as the time of elution of the peptide of interest from the liquid chromatography column. In particular, the chromatographic data is acquired during selective reaction monitoring of an eluate from the liquid chromatography column containing the peptide of interest. The chromatographic data includes ion signals measured along the common chromatographic time scale for each of the plurality of candidate fragment ions.

Abstract translation: 用于从液相色谱柱确定感兴趣的肽的时间的方法包括获得感兴趣的肽的多个候选碎片离子中的每一个的色谱数据的步骤。 沿着常见色谱时间的时间是对应于针对多个候选碎片离子中的每一个测量的离子信号的最大重叠而确定的刻度。 最后，确定的时间被指定为目的肽从液相色谱柱洗脱的时间。 特别地，在从含有感兴趣的肽的液相色谱柱的洗脱液的选择性反应监测期间获取色谱数据。 色谱数据包括沿着多个候选碎片离子中的每一个的公共色谱时间标度测量的离子信号。