公开(公告)号：US20240361332A1

公开(公告)日：2024-10-31

申请号：US18764888

申请日：2024-07-05

申请人： FUJIFILM Corporation

发明人： Shun MATSUDA

IPC分类号： G01N33/68 ,  G01N33/58

CPC分类号： G01N33/6848 ,  G01N33/58 ,  G01N2333/015

摘要： An object of the present invention is to provide a method of identifying an amino acid site in a protein, to which a sugar is bound more accurately than in the related art, and a kit for performing the method. According to the present invention, there is provided a method for identifying an amino acid site in a protein, to which a sugar is bonded, the method including a first mass spectrometry step of subjecting a fragmented protein to mass spectrometry; for a protein in which the bonding of the sugar has been confirmed in the first mass spectrometry step, a substitution-modification step of substituting and/or modifying an amino acid of the protein at the amino acid site in the protein, to which the sugar is bonded and a fragmentation step of fragmenting the protein; and a second mass spectrometry step of subjecting the substituted and/or modified, and fragmented protein obtained by the substitution-modification step and the fragmentation step, to mass spectrometry.

公开(公告)号：US20240361307A1

公开(公告)日：2024-10-31

申请号：US18606701

申请日：2024-03-15

申请人： THE BRIGHAM AND WOMEN'S HOSPITAL, INC.

发明人： Omid Farokhzad ,  Morteza Mahmoudi ,  Claudia Corbo

IPC分类号： G01N33/543 ,  C01G49/02 ,  G01N33/574 ,  G01N33/68 ,  G06F18/24 ,  G06N20/20 ,  G16B40/20 ,  B82Y35/00

CPC分类号： G01N33/5432 ,  C01G49/02 ,  G01N33/54326 ,  G01N33/57488 ,  G01N33/6842 ,  G01N33/6848 ,  G06F18/24 ,  G06N20/20 ,  G16B40/20 ,  B82Y35/00 ,  C01P2004/64 ,  G06F2218/20

摘要： The present disclosure provides a system comprising a communication interface and computer for assigning a label to the biomolecule fingerprint, wherein the label corresponds to a biological state. The present disclosure also provides a sensor arrays for detecting biomolecules and methods of use. In some embodiments, the sensor arrays are capable of determining a disease state in a subject.

公开(公告)号：US20240360176A1

公开(公告)日：2024-10-31

申请号：US18635162

申请日：2024-04-15

申请人： Marshall University Research Corporation

发明人： Juan Sanabria ,  Sandrine Pierre ,  Moumita Banerjee ,  Zijian Xie ,  Joseph Shapiro

IPC分类号： C07K7/08 ,  A61K38/00 ,  C12Q1/6886 ,  G01N33/574 ,  G01N33/68

CPC分类号： C07K7/08 ,  C12Q1/6886 ,  G01N33/57438 ,  G01N33/6848 ,  A61K38/00 ,  C07K2319/10 ,  G01N2333/4703 ,  G01N2800/52

摘要： Methods for treating non-alcoholic steatohepatitis and/or hepatocellular carcinoma include administering a polypeptide antagonist of a Na/K ATPase/Src receptor complex to a subject in need thereof. Methods and assays for diagnosis or prognosis of non-alcoholic steatohepatitis and/or hepatocellular carcinoma in a subject are also provided and include the steps of providing a biological sample from the subject, determining an expression level or activity in the sample of at least one biomarker selected from Caveolin-1, Survivin, and SMAC; and comparing the expression level or activity of the at least one biomarker in the sample, if present, to a control expression level or activity of the at least one biomarker. Prophylaxis or treatment of the non-alcoholic steatohepatitis and/or hepatocellular carcinoma in a subject can then be initiated based on the expression level or activity of Caveolin-1, Survivin, and SMAC in the sample.

公开(公告)号：US20240345086A1

公开(公告)日：2024-10-17

申请号：US18579635

申请日：2022-07-20

申请人： UCL Business LTD

发明人： Kevin Mills ,  Wendy Heywood ,  Ivan Doykov ,  James Moon

IPC分类号： G01N33/569 ,  G01N33/68

CPC分类号： G01N33/56983 ,  G01N33/6848 ,  G01N2333/165 ,  G01N2469/20

摘要： The present invention relates to a high throughput targeted proteomics-based method for identifying and quantifying one or more binding molecules in a biological fluid of an individual. The present invention involves the use of a plurality of bait molecules to capture the binding molecules. The invention uses proteomics techniques, such as mass spectrometry, for the identification and quantification of the binding molecules. The methods of the invention can be used to identify and quantify antibodies produced by an individual, and proteins expressed by an individual, following exposure to antigens, such as viral antigens, particularly coronavirus antigens and more particularly SARS-CoV-2. Exposure may be via vaccination or following natural infection. The methods of the invention can be used to identify and quantify molecules which bind to a range of proteins of interest, such as autoantigens and neoantigens, as well as to viral vectors.

公开(公告)号：US12117452B2

公开(公告)日：2024-10-15

申请号：US16850363

申请日：2020-04-16

申请人： Regeneron Pharmaceuticals, Inc.

发明人： I-Hsuan Chen ,  Hui Xiao ,  Ning Li

IPC分类号： G01N33/68 ,  G01N1/40

CPC分类号： G01N33/6848 ,  G01N1/4005 ,  G01N2458/15 ,  G01N2560/00

摘要： Method of identifying host-cell proteins in a sample matrix are provided.

公开(公告)号：US12110314B2

公开(公告)日：2024-10-08

申请号：US17955214

申请日：2022-09-28

申请人： Lawrence Livermore National Security, LLC

发明人： Mathew Gerald Lyman ,  Deon S. Anex ,  Bonnee Rubinfeld

IPC分类号： C07K14/245 ,  G01N33/68

CPC分类号： C07K14/245 ,  G01N33/6848

摘要： Described are methods, compositions, and devices for a concatemeric protein standard that behaves as a protein but transforms into single peptides upon digestion, which is optimized to function as a non-obtrusive process control for mass spectrometry analysis.

公开(公告)号：US12110309B2

公开(公告)日：2024-10-08

申请号：US16590676

申请日：2019-10-02

申请人： SHIMADZU CORPORATION

发明人： Takashi Nishikaze

IPC分类号： C07H1/00 ,  G01N33/68

CPC分类号： C07H1/00 ,  G01N33/6848 ,  G01N2400/00 ,  G01N2440/38 ,  G01N2560/00

摘要： A method for preparing an analytical sample for analysis of a glycan contained in a sample includes: performing an amidation reaction that amidates a lactone structure included in the glycan through contacting the sample with a reaction solution that is basic; adding an acidic solution to the reaction solution after the reaction solution is subjected to the amidation reaction; and purifying the sample contained in the reaction solution after the acidic solution is added to the reaction solution by using a carrier for hydrophilic interaction chromatography.

公开(公告)号：US20240319203A1

公开(公告)日：2024-09-26

申请号：US18579620

申请日：2022-08-04

申请人： H. LUNDBECK A/S

发明人： Anne-Marie JACOBSEN ,  Line Rorbaek OLSEN ,  Frank SCHALK ,  Nico van de MERBEL

IPC分类号： G01N33/68

CPC分类号： G01N33/6848 ,  G01N33/6842 ,  G01N33/6896 ,  G01N2800/2821 ,  G01N2800/52

摘要： The present invention relates to a method of measuring pS396 Tau in a sample comprising the steps of i-iv, i) Treating a CSF sample from a subject suffering or suspected to be suffering from a Tau pathology with trypsin, ii) Subjecting the sample in step i) to a desphosphorylation agent and subsequently take part of the sample aside for step iv) before proceeding to step iii) with the remaining sample, iii) Subjecting the dephosphorylated sample from step ii) to a second trypsin treatment, iv) Measuring the amount of the Tau peptides (SPVVSGDTSPR) corresponding to Tau residues 396-406 in the samples from step ii) and step iii) using LC-MS.

公开(公告)号：US20240316223A1

公开(公告)日：2024-09-26

申请号：US18731289

申请日：2024-06-01

申请人： The Regents of the University of California

发明人： Patrick J. Moon ,  Xaio Xie ,  F. Dean Toste ,  Christopher J. Chang

IPC分类号： A61K51/04 ,  G01N33/68

CPC分类号： A61K51/0406 ,  G01N33/6812 ,  G01N33/6848 ,  G01N2570/00

摘要： A redox-based strategy for bioconjugation of tryptophan uses oxaziridine reagents that mimic oxidative cyclization reactions in indole-based alkaloid biosynthetic pathways to achieve highly selective and rapid tryptophan labeling.

公开(公告)号：US20240295564A1

公开(公告)日：2024-09-05

申请号：US18575108

申请日：2022-06-28

申请人： ETH ZURICH

发明人： Xiaobao CAO ,  Peng XUE ,  Rudolf AEBERSOLD ,  Stavros STAVRAKIS ,  Andrew James DEMELLO

IPC分类号： G01N33/68 ,  B01L3/00

CPC分类号： G01N33/6827 ,  B01L3/502761 ,  G01N33/6845 ,  G01N33/6848 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/087 ,  B01L2400/0633

摘要： In a method for analyzing cells, a sample fluid having a suspending medium and cells is fed to a microfluidic device having at least one cell processing unit having a trapping region, a reaction unit, and an outlet arrangement. The trapping region is delimited by at least an input valve and a sieve valve, in particular a v-type valve that is capable of retaining the cells while letting fluids pass. The method includes trapping cells in the trapping region, subsequently establishing a flow of a reaction fluid through the trapping region while the sieve valve assumes the open state, such that the reaction fluid transfers the trapped cells from the trapping region into the reaction unit, decomposing the transferred cells into cell fragments through a decomposition process, collecting the cell fragments in the outlet arrangement, and analyzing the cell fragments.