公开(公告)号：US20210156813A1

公开(公告)日：2021-05-27

申请号：US16914682

申请日：2020-06-29

申请人： Board of Regents, The University of Texas System

发明人： Arjang Hassibi ,  Arun Manickam ,  Rituraj Singh

IPC分类号： G01N27/27 ,  C12Q1/6874 ,  G01N27/327

摘要： A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.

公开(公告)号：US20180335399A1

公开(公告)日：2018-11-22

申请号：US15961401

申请日：2018-04-24

申请人： Board of Regents, The University of Texas System

发明人： Arjang Hassibi ,  Arun Manickam ,  Rituraj Singh

IPC分类号： G01N27/327

CPC分类号： G01N27/3277

摘要： A biosensor pixel for measuring current that flows through the electrode surface in response to electrochemical interactions and a biosensor array architecture that includes such biosensor pixels. The biosensor pixel includes an electrode transducer configured to measure a current generated by electrochemical interactions occurring at a recognition layer placed directly on top of it in response to an electrical voltage placed across an electrode transducer-electrolyte interface. The biosensor pixel further includes a trans-impedance amplifier connected to the electrode transducer, where the trans-impedance amplifier is configured to convert the current into a voltage signal as the electrochemical interactions occur. Additionally, the biosensor pixel includes a 1-bit comparator coupled to the trans-impedance amplifier and a 1-bit digital-to-analog converter coupled to the 1-bit comparator, where the 1-bit digital-to-analog converter injects different levels of charge into an input of the trans-impedance amplifier at each cycle based on an output of the 1-bit comparator.

公开(公告)号：US09465002B2

公开(公告)日：2016-10-11

申请号：US13959492

申请日：2013-08-05

申请人： Board of Regents, The University of Texas System

发明人： Arjang Hassibi ,  Arun Manickam ,  Rituraj Singh

IPC分类号： G01N27/00 ,  G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N27/27 ,  G01N27/327 ,  C12Q1/68

CPC分类号： G01N27/27 ,  C12Q1/6874 ,  G01N27/3276 ,  C12Q2535/122 ,  C12Q2565/607

摘要： A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.

摘要翻译： 用于进行DNA测序并从未知核酸链提取结构信息的方法和装置。 该装置包括微孔结构，其中相同的DNA链固定在微孔结构内的微珠，活性电极或多孔聚合物的表面上。 该器件还包括CMOS集成半导体集成电路，其中CMOS集成半导体集成电路在硅衬底上包括金属层，其中金属层形成有源电极生物传感器。 此外，通过在CMOS集成半导体集成电路的钝化层中形成开口以形成固定有DNA链的单个珠，形成感测电极。