公开(公告)号：US10739293B2

公开(公告)日：2020-08-11

申请号：US15097037

申请日：2016-04-12

申请人： Board of Regents, The University of Texas System

发明人： Arjang Hassibi ,  Arun Manickam ,  Rituraj Singh

IPC分类号： C12P19/34 ,  G01N27/27 ,  C12Q1/6874 ,  G01N27/327

摘要： A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.

公开(公告)号：US09983163B2

公开(公告)日：2018-05-29

申请号：US13873684

申请日：2013-04-30

申请人： Board of Regents, The University of Texas System

发明人： Arjang Hassibi ,  Arun Manickam ,  Rituraj Singh

IPC分类号： G01N27/327

CPC分类号： G01N27/3277

摘要： A biosensor pixel for measuring current that flows through the electrode surface in response to electrochemical interactions and a biosensor array architecture that includes such biosensor pixels. The biosensor pixel includes an electrode transducer configured to measure a current generated by electrochemical interactions occurring at a recognition layer placed directly on top of it in response to an electrical voltage placed across an electrode transducer-electrolyte interface. The biosensor pixel further includes a trans-impedance amplifier connected to the electrode transducer, where the trans-impedance amplifier is configured to convert the current into a voltage signal as the electrochemical interactions occur. Additionally, the biosensor pixel includes a 1-bit comparator coupled to the trans-impedance amplifier and a 1-bit digital-to-analog converter coupled to the 1-bit comparator, where the 1-bit digital-to-analog converter injects different levels of charge into an input of the trans-impedance amplifier at each cycle based on an output of the 1-bit comparator.

公开(公告)号：US20160231270A1

公开(公告)日：2016-08-11

申请号：US15097037

申请日：2016-04-12

申请人： Board of Regents, The University of Texas System

发明人： Arjang Hassibi ,  Arun Manickam ,  Rituraj Singh

IPC分类号： G01N27/327 ,  G01N27/27 ,  C12Q1/68

CPC分类号： G01N27/27 ,  C12Q1/6874 ,  G01N27/3276 ,  C12Q2535/122 ,  C12Q2565/607

摘要： A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.

公开(公告)号：US20140318958A1

公开(公告)日：2014-10-30

申请号：US13873684

申请日：2013-04-30

申请人： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM

发明人： Arjang Hassibi ,  Arun Manickam ,  Rituraj Singh

IPC分类号： G01N27/414

CPC分类号： G01N27/3277

摘要： A biosensor pixel for measuring current that flows through the electrode surface in response to electrochemical interactions and a biosensor array architecture that includes such biosensor pixels. The biosensor pixel includes an electrode transducer configured to measure a current generated by electrochemical interactions occurring at a recognition layer placed directly on top of it in response to an electrical voltage placed across an electrode transducer-electrolyte interface. The biosensor pixel further includes a trans-impedance amplifier connected to the electrode transducer, where the trans-impedance amplifier is configured to convert the current into a voltage signal as the electrochemical interactions occur. Additionally, the biosensor pixel includes a 1-bit comparator coupled to the trans-impedance amplifier and a 1-bit digital-to-analog converter coupled to the 1-bit comparator, where the 1-bit digital-to-analog converter injects different levels of charge into an input of the trans-impedance amplifier at each cycle based on an output of the 1-bit comparator.

摘要翻译： 一种用于测量响应于电化学相互作用而流过电极表面的电流的生物传感器像素和包括这种生物传感器像素的生物传感器阵列结构。 生物传感器像素包括电极换能器，其被配置为响应于放置在电极换能器 - 电解质界面上的电压来测量在直接位于其顶部的识别层上发生的电化学相互作用产生的电流。 生物传感器像素还包括连接到电极换能器的跨阻抗放大器，其中跨阻放大器被配置为随着电化学相互作用发生而将电流转换成电压信号。 此外，生物传感器像素包括耦合到跨阻抗放大器的1位比较器和耦合到1位比较器的1位数模转换器，其中1位数模转换器注入不同的 基于1位比较器的输出，每个周期的跨阻抗放大器输入的电荷电平。

公开(公告)号：US11579111B2

公开(公告)日：2023-02-14

申请号：US15961401

申请日：2018-04-24

申请人： Board of Regents, The University of Texas System

发明人： Arjang Hassibi ,  Arun Manickam ,  Rituraj Singh

IPC分类号： G01N27/327

摘要： A biosensor pixel for measuring current that flows through the electrode surface in response to electrochemical interactions and a biosensor array architecture that includes such biosensor pixels. The biosensor pixel includes an electrode transducer configured to measure a current generated by electrochemical interactions occurring at a recognition layer placed directly on top of it in response to an electrical voltage placed across an electrode transducer-electrolyte interface. The biosensor pixel further includes a trans-impedance amplifier connected to the electrode transducer, where the trans-impedance amplifier is configured to convert the current into a voltage signal as the electrochemical interactions occur. Additionally, the biosensor pixel includes a 1-bit comparator coupled to the trans-impedance amplifier and a 1-bit digital-to-analog converter coupled to the 1-bit comparator, where the 1-bit digital-to-analog converter injects different levels of charge into an input of the trans-impedance amplifier at each cycle based on an output of the 1-bit comparator.

公开(公告)号：US20140011710A1

公开(公告)日：2014-01-09

申请号：US13959492

申请日：2013-08-05

申请人： Board of Regents, The University of Texas System

发明人： Arjang Hassibi ,  Arun Manickam ,  Rituraj Singh

IPC分类号： G01N27/27

CPC分类号： G01N27/27 ,  C12Q1/6874 ,  G01N27/3276 ,  C12Q2535/122 ,  C12Q2565/607

摘要： A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.

摘要翻译： 用于进行DNA测序并从未知核酸链提取结构信息的方法和装置。 该装置包括微孔结构，其中相同的DNA链固定在微孔结构内的微珠，活性电极或多孔聚合物的表面上。 该器件还包括CMOS集成半导体集成电路，其中CMOS集成半导体集成电路在硅衬底上包括金属层，其中金属层形成有源电极生物传感器。 此外，通过在CMOS集成半导体集成电路的钝化层中形成开口以形成固定有DNA链的单个珠，形成感测电极。

公开(公告)号：US20230333039A1

公开(公告)日：2023-10-19

申请号：US17876871

申请日：2022-07-29

申请人： Board of Regents, The University of Texas System

发明人： Arjang Hassibi ,  Arun Manickam ,  Rituraj Singh

IPC分类号： G01N27/27 ,  C12Q1/6874 ,  G01N27/327

CPC分类号： G01N27/27 ,  C12Q1/6874 ,  G01N27/3276

摘要： A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.

公开(公告)号：US20230141046A1

公开(公告)日：2023-05-11

申请号：US18095716

申请日：2023-01-11

申请人： Board of Regents, The University of Texas System

发明人： Arjang Hassibi ,  Arun Manickam ,  Rituraj Singh

IPC分类号： G01N27/327

CPC分类号： G01N27/3277

摘要： A biosensor pixel for measuring current that flows through the electrode surface in response to electrochemical interactions and a biosensor array architecture that includes such biosensor pixels. The biosensor pixel includes an electrode transducer configured to measure a current generated by electrochemical interactions occurring at a recognition layer placed directly on top of it in response to an electrical voltage placed across an electrode transducer-electrolyte interface. The biosensor pixel further includes a trans-impedance amplifier connected to the electrode transducer, where the trans-impedance amplifier is configured to convert the current into a voltage signal as the electrochemical interactions occur. Additionally, the biosensor pixel includes a 1-bit comparator coupled to the trans-impedance amplifier and a 1-bit digital-to-analog converter coupled to the 1-bit comparator, where the 1-bit digital-to-analog converter injects different levels of charge into an input of the trans-impedance amplifier at each cycle based on an output of the 1-bit comparator.

公开(公告)号：US20220373495A1

公开(公告)日：2022-11-24

申请号：US17558084

申请日：2021-12-21

申请人： Board of Regents, The University of Texas System

发明人： Arjang Hassibi ,  Arun Manickam ,  Rituraj Singh

IPC分类号： G01N27/27 ,  C12Q1/6874 ,  G01N27/327

摘要： A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.

公开(公告)号：US20220099613A1

公开(公告)日：2022-03-31

申请号：US17171820

申请日：2021-02-09

申请人： Board of Regents, The University of Texas System

发明人： Arjang Hassibi ,  Arun Manickam ,  Rituraj Singh

IPC分类号： G01N27/27 ,  C12Q1/6874 ,  G01N27/327

摘要： A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.