公开(公告)号：US11061039B2

公开(公告)日：2021-07-13

申请号：US16208739

申请日：2018-12-04

Applicant: CLEVELAND HEARTLAB, INC.

Inventor： Timothy Collier ,  Cory Bystrom ,  Angela Higgins

IPC: G01N33/483 ,  G01N33/92 ,  G01N1/34 ,  C07K14/775

Abstract: The present invention provides methods, kits, and compositions for purifying HDL molecules from a sample (e.g., blood sample) using HDL tagging molecules comprising an HDL lipophilic core binding peptide (e.g., portion of ApoA1) and an affinity tag. The present invention also provides methods, kits, and compositions for detecting non-fragmented ApoA1 with mass spectrometry. The present invention further provides methods, kits, and compositions for tagging HDL molecules in a sample with detectably labeled ApoA1 molecules such that the ratio of detectably labeled ApoA1 molecules to native ApoA1 proteins may be determined.

公开(公告)号：US10921331B2

公开(公告)日：2021-02-16

申请号：US15656592

申请日：2017-07-21

Applicant: Cleveland HeartLab, Inc.

Inventor： Cory Bystrom ,  Timothy Collier

IPC: G01N33/92

Abstract: Provided herein are methods, systems, and compositions for detecting one or more HDL-associated proteins (e.g., ApoC3; ApoC3 and ApoA1; ApoC3 and SAA1/2; or proteins in Biomarker Panels 1-30) in a sample from a subject with, or suspected of having, cardiovascular disease (CVD) or other HDL related disease. In certain embodiments, such methods, systems, and compositions are used to determine the approximate risk of CVD (or other disease) for a subject, and/or the approximate cholesterol efflux capacity (CEC) of a sample.

公开(公告)号：US10466260B2

公开(公告)日：2019-11-05

申请号：US15788876

申请日：2017-10-20

Applicant: Cleveland HeartLab, Inc.

Inventor： Cory Bystrom ,  Timothy Collier

IPC: A61K38/00 ,  G01N33/92

Abstract: Provided herein are compositions, systems, and methods for extracting and detecting at least one HDL-associated protein (e.g., ApoA1) from a sample (e.g., plasma or serum sample). In certain embodiments, a strong organic acid and hydrophilic organic solvent are mixed with the sample; after centrifugation, the supernatant is transferred to a second container where it is mixed with a non-polar organic solvent; after centrifugation, the lower aqueous layer is transferred to a third container; and then at least a portion of the transferred aqueous layer is subjected to a detection assay such that at least one HDL-associated protein is detected.

公开(公告)号：US20180031586A1

公开(公告)日：2018-02-01

申请号：US15788876

申请日：2017-10-20

Applicant: Cleveland HeartLab, Inc.

Inventor： Cory Bystrom ,  Timothy Collier

IPC: G01N33/92

CPC classification number: G01N33/92 ,  G01N2333/775

Abstract: Provided herein are compositions, systems, and methods for extracting and detecting at least one HDL-associated protein (e.g., ApoA1) from a sample (e.g., plasma or serum sample). In certain embodiments, a strong organic acid and hydrophilic organic solvent are mixed with the sample; after centrifugation, the supernatant is transferred to a second container where it is mixed with a non-polar organic solvent; after centrifugation, the lower aqueous layer is transferred to a third container; and then at least a portion of the transferred aqueous layer is subjected to a detection assay such that at least one HDL-associated protein is detected.

公开(公告)号：US20160084856A1

公开(公告)日：2016-03-24

申请号：US14858111

申请日：2015-09-18

Applicant: Cleveland HeartLab, Inc.

Inventor： Cory Bystrom ,  Timothy Collier

IPC: G01N33/92

CPC classification number: G01N33/92 ,  G01N2333/775

Abstract: Provided herein are compositions, systems, and methods for extracting and detecting at least one HDL-associated protein (e.g., ApoA1) from a sample (e.g., plasma or serum sample). In certain embodiments, a strong organic acid and hydrophilic organic solvent are mixed with the sample; after centrifugation, the supernatant is transferred to a second container where it is mixed with a non-polar organic solvent; after centrifugation, the lower aqueous layer is transferred to a third container; and then at least a portion of the transferred aqueous layer is subjected to a detection assay such that at least one HDL-associated protein is detected.

Abstract translation: 本文提供了用于从样品（例如血浆或血清样品）提取和检测至少一种HDL相关蛋白（例如ApoA1）的组合物，系统和方法。 在某些实施方案中，将强有机酸和亲水性有机溶剂与样品混合; 离心后，将上清转移到第二容器中，在其中与非极性有机溶剂混合; 离心后，将下层水层转移到第三容器中; 然后将至少一部分转移的水层进行检测测定，使得至少检测到一种HDL相关蛋白。