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    COMPOSITIONS AND METHODS FOR PURIFICATION AND DETECTION OF HDL AND APOAI
    1.
    发明申请
    COMPOSITIONS AND METHODS FOR PURIFICATION AND DETECTION OF HDL AND APOAI 有权

    公开(公告)号：US20250027960A1

    公开(公告)日：2025-01-23

    申请号：US18829111

    申请日：2024-09-09

    Applicant: CLEVELAND HEARTLAB, INC.

    Inventor： Timothy Collier ,  Cory Bystrom ,  Angela Higgins

    IPC: G01N33/92 ,  C07K14/775 ,  G01N1/34

    Abstract: The present invention provides methods, kits, and compositions for purifying HDL molecules from a sample (e.g., blood sample) using HDL tagging molecules comprising an HDL lipophilic core binding peptide (e.g., portion of ApoA1) and an affinity tag. The present invention also provides methods, kits, and compositions for detecting non-fragmented ApoA1 with mass spectrometry. The present invention further provides methods, kits, and compositions for tagging HDL molecules in a sample with detectably labeled ApoA1 molecules such that the ratio of detectably labeled ApoA1 molecules to native ApoA1 proteins may be determined.

    HDL-ASSOCIATED PROTEIN BIOMARKER PANEL DETECTION
    3.
    发明申请
    HDL-ASSOCIATED PROTEIN BIOMARKER PANEL DETECTION 有权

    公开(公告)号：US20210132090A1

    公开(公告)日：2021-05-06

    申请号：US17143463

    申请日：2021-01-07

    Applicant: Cleveland Heartlab, Inc.

    Inventor： Cory Bystrom ,  Timothy Collier

    IPC: G01N33/92

    Abstract: Provided herein are methods, systems, and compositions for detecting one or more HDL-associated proteins (e.g., ApoC3; ApoC3 and ApoA1; ApoC3 and SAA1/2; or proteins in Biomarker Panels 1-30) in a sample from a subject with, or suspected of having, cardiovascular disease (CVD) or other HDL related disease. In certain embodiments, such methods, systems, and compositions are used to determine the approximate risk of CVD (or other disease) for a subject, and/or the approximate cholesterol efflux capacity (CEC) of a sample.

    COMPOSITIONS AND METHODS FOR PURIFICATION AND DETECTION OF HDL AND APOA1
    4.
    发明申请
    COMPOSITIONS AND METHODS FOR PURIFICATION AND DETECTION OF HDL AND APOA1 审中-公开
    用于纯化和检测HDL和APOA1的组合物和方法

    公开(公告)号：US20150331000A1

    公开(公告)日：2015-11-19

    申请号：US14713046

    申请日：2015-05-15

    Applicant: CLEVELAND HEARTLAB, INC.

    Inventor： Timothy Collier ,  Cory Bystrom ,  Angela Higgins

    IPC: G01N33/92 ,  G01N1/34

    CPC classification number: G01N33/92 ,  C07K14/775 ,  G01N1/34

    Abstract: The present invention provides methods, kits, and compositions for purifying HDL molecules from a sample (e.g., blood sample) using HDL tagging molecules comprising an HDL lipophilic core binding peptide (e.g., portion of ApoA1) and an affinity tag. The present invention also provides methods, kits, and compositions for detecting non-fragmented ApoA1 with mass spectrometry. The present invention further provides methods, kits, and compositions for tagging HDL molecules in a sample with detectably labeled ApoA1 molecules such that the ratio of detectably labeled ApoA1 molecules to native ApoA1 proteins may be determined.

    Abstract translation: 本发明提供了使用包含HDL亲脂性核心结合肽（例如，ApoA1的部分）和亲和标签的HDL标记分子从样品（例如血液样品）中纯化HDL分子的方法，试剂盒和组合物。 本发明还提供用质谱法检测非碎片化的ApoA1的方法，试剂盒和组合物。 本发明还提供用于标记具有可检测标记的ApoA1分子的样品中的HDL分子的方法，试剂盒和组合物，使得可测定可检测标记的ApoA1分子与天然ApoA1蛋白的比例。

    HDL-associated protein extraction and detection
    6.
    发明授权
    HDL-associated protein extraction and detection 有权

    公开(公告)号：US11940452B2

    公开(公告)日：2024-03-26

    申请号：US16667016

    申请日：2019-10-29

    Applicant: CLEVELAND HEARTLAB, INC.

    Inventor： Cory Bystrom ,  Timothy Collier

    IPC: G01N33/92

    CPC classification number: G01N33/92 ,  G01N2333/775

    Abstract: Provided herein are compositions, systems, and methods for extracting and detecting at least one HDL-associated protein (e.g., ApoA1) from a sample (e.g., plasma or serum sample). In certain embodiments, a strong organic acid and hydrophilic organic solvent are mixed with the sample; after centrifugation, the supernatant is transferred to a second container where it is mixed with a non-polar organic solvent; after centrifugation, the lower aqueous layer is transferred to a third container; and then at least a portion of the transferred aqueous layer is subjected to a detection assay such that at least one HDL-associated protein is detected.

    COMPOSITIONS AND METHODS FOR PURIFICATION AND DETECTION OF HDL AND APOAI
    7.
    发明申请
    COMPOSITIONS AND METHODS FOR PURIFICATION AND DETECTION OF HDL AND APOAI 有权

    公开(公告)号：US20210373038A1

    公开(公告)日：2021-12-02

    申请号：US17367049

    申请日：2021-07-02

    Applicant: CLEVELAND HEARTLAB, INC.

    Inventor： Timothy Collier ,  Cory Bystrom ,  Angela Higgins

    IPC: G01N33/92 ,  G01N1/34

    Abstract: The present invention provides methods, kits, and compositions for purifying HDL molecules from a sample (e.g., blood sample) using HDL tagging molecules comprising an HDL lipophilic core binding peptide (e.g., portion of ApoA1) and an affinity tag. The present invention also provides methods, kits, and compositions for detecting non-fragmented ApoA1 with mass spectrometry. The present invention further provides methods, kits, and compositions for tagging HDL molecules in a sample with detectably labeled ApoA1 molecules such that the ratio of detectably labeled ApoA1 molecules to native ApoA1 proteins may be determined.

    Compositions and methods for purification and detection of HDL and ApoA1
    8.
    发明授权
    Compositions and methods for purification and detection of HDL and ApoA1 有权

    公开(公告)号：US10151764B2

    公开(公告)日：2018-12-11

    申请号：US14713046

    申请日：2015-05-15

    Applicant: CLEVELAND HEARTLAB, INC.

    Inventor： Timothy Collier ,  Cory Bystrom ,  Angela Higgins

    IPC: G01N33/483 ,  G01N33/92 ,  G01N1/34 ,  C07K14/775

    Abstract: The present invention provides methods, kits, and compositions for purifying HDL molecules from a sample (e.g., blood sample) using HDL tagging molecules comprising an HDL lipophilic core binding peptide (e.g., portion of ApoA1) and an affinity tag. The present invention also provides methods, kits, and compositions for detecting non-fragmented ApoA1 with mass spectrometry. The present invention further provides methods, kits, and compositions for tagging HDL molecules in a sample with detectably labeled ApoA1 molecules such that the ratio of detectably labeled ApoA1 molecules to native ApoA1 proteins may be determined.

    HDL-ASSOCIATED PROTEIN BIOMARKER PANEL DETECTION
    9.
    发明申请
    HDL-ASSOCIATED PROTEIN BIOMARKER PANEL DETECTION 审中-公开

    公开(公告)号：US20180024152A1

    公开(公告)日：2018-01-25

    申请号：US15656592

    申请日：2017-07-21

    Applicant: Cleveland HeartLab, Inc.

    Inventor： Cory Bystrom ,  Timothy Collier

    IPC: G01N33/92

    Abstract: Provided herein are methods, systems, and compositions for detecting one or more HDL-associated proteins (e.g., ApoC3; ApoC3 and ApoA1; ApoC3 and SAA1/2; or proteins in Biomarker Panels 1-30) in a sample from a subject with, or suspected of having, cardiovascular disease (CVD) or other HDL related disease. In certain embodiments, such methods, systems, and compositions are used to determine the approximate risk of CVD (or other disease) for a subject, and/or the approximate cholesterol efflux capacity (CEC) of a sample.

    HDL-associated protein extraction and detection
    10.
    发明授权
    HDL-associated protein extraction and detection 有权

    公开(公告)号：US09810702B2

    公开(公告)日：2017-11-07

    申请号：US14858111

    申请日：2015-09-18

    Applicant: Cleveland HeartLab, Inc.

    Inventor： Cory Bystrom ,  Timothy Collier

    IPC: G01N33/92

    CPC classification number: G01N33/92 ,  G01N2333/775

    Abstract: Provided herein are compositions, systems, and methods for extracting and detecting at least one HDL-associated protein (e.g., ApoA1) from a sample (e.g., plasma or serum sample). In certain embodiments, a strong organic acid and hydrophilic organic solvent are mixed with the sample; after centrifugation, the supernatant is transferred to a second container where it is mixed with a non-polar organic solvent; after centrifugation, the lower aqueous layer is transferred to a third container; and then at least a portion of the transferred aqueous layer is subjected to a detection assay such that at least one HDL-associated protein is detected.

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