公开(公告)号：US20170095503A1

公开(公告)日：2017-04-06

申请号：US15291849

申请日：2016-10-12

Applicant: DUKE UNIVERSITY

Inventor： Bruce A. Sullenger ,  Jaewoo Lee

IPC: A61K31/785 ,  A61K31/13 ,  A61K31/722

CPC classification number: A61K31/785 ,  A61K31/13 ,  A61K31/70 ,  A61K31/722 ,  A61K38/16 ,  A61K38/1709

Abstract: The present invention relates, in general, to pattern-recognition receptors (PRRs), including toll-like receptors (TLRs), and, in particular, to a method of inhibiting nucleic acid-induced activation of, for example, endosomal TLRs using an agent that binds to the nucleic acid (“nucleic acid binding agent”), preferably, in a manner that is independent of the nucleotide sequence, the chemistry (e.g., DNA or RNA, with or without base or sugar modifications) and/or the structure (e.g., double-stranded or single-stranded, complexed or uncomplexed with, for example protein) of the nucleic acid(s) responsible for inducing TLR activation. The invention also relates to methods of identifying nucleic acid binding agents suitable for use in such methods.

公开(公告)号：US20160030332A1

公开(公告)日：2016-02-04

申请号：US14783717

申请日：2014-04-09

Applicant: DUKE UNIVERSITY

Inventor： Jaewoo Lee ,  Johannes Urban ,  Bruce A. Sullenger

IPC: A61K9/00 ,  A61K9/127 ,  A61K31/7115 ,  A61K39/00 ,  C12N15/117 ,  C12N15/115

CPC classification number: A61K9/0012 ,  A61K9/127 ,  A61K31/7115 ,  A61K39/0011 ,  C12N15/115 ,  C12N15/117 ,  C12N2310/16 ,  C12N2310/17 ,  C12N2310/31 ,  C12N2310/33 ,  C12N2310/335 ,  C12N2310/531 ,  C12N2320/31 ,  C12N2320/32 ,  C12N2310/322 ,  C12N2310/3533

Abstract: Methods of inhibiting the growth of cells or inducing cell death by contacting the cells with or introducing into the cells a composition including a 5′ triphosphate, 2′ fluoro-modified pyrimidine non-linear single stranded RNA at least 17 nucleotides long with a least 3 base pairings or a 5′ triphosphate, 2′ fluoro-modified double stranded RNA at least 17 base pairs long in an amount effective to inhibit cell growth, induce cell death or induce cytokine production by the cells. The methods also include administration of the compositions to a subject. The subject may have a proliferative disorder or infectious disease and administration of the compositions provided herein may treat the disorder or disease.

Abstract translation: 通过使细胞与细胞接触或引入细胞来抑制细胞生长或诱导细胞死亡的方法包括至少17个核苷酸长的5'三磷酸，2'氟修饰的嘧啶非线性单链RNA的组合物，具有至少3个 碱基配对或5'三磷酸，2'氟修饰的双链RNA，至少17碱基对长，有效抑制细胞生长，诱导细胞死亡或诱导细胞产生细胞因子。 所述方法还包括将组合物给予受试者。 受试者可能具有增殖性疾病或感染性疾病，并且本文提供的组合物的施用可以治疗疾病或疾病。