公开(公告)号：US20140287015A1

公开(公告)日：2014-09-25

申请号：US14343150

申请日：2012-09-07

申请人： Edward H. Schuchman ,  Calogera M. Simonaro

发明人： Edward H. Schuchman ,  Calogera M. Simonaro

IPC分类号： A61K38/50 ,  A61K35/32 ,  C12N5/077

CPC分类号： A61K38/50 ,  A61K35/32 ,  A61K38/1841 ,  C12N5/0655 ,  C12N2501/73 ,  C12N2506/1353 ,  C12Y305/01023 ,  A61K2300/00

摘要： The present invention relates to methods of producing chondrocytes, improving the phenotype of a chondrocyte population, promoting chondrogenesis, maintaining a cell population in a differentiated state or increasing the number of cells of a population in a differentiated state, producing a population of differentiated cells, and treating certain diseases or disorders. Each of these methods involves treating a cell population with, and/or culturing a cell population in, a ceramidase

摘要翻译： 本发明涉及生产软骨细胞的方法，改善软骨细胞群体的表型，促进软骨形成，维持细胞群处于分化状态或增加分化状态的群体的细胞数，产生分化细胞群体， 并治疗某些疾病或病症。 这些方法中的每一种涉及用神经酰胺酶处理细胞群和/或培养细胞群

公开(公告)号：US20120115158A1

公开(公告)日：2012-05-10

申请号：US13318377

申请日：2010-05-03

申请人： Edward H. Schuchman ,  Efrat Eliyahu

发明人： Edward H. Schuchman ,  Efrat Eliyahu

IPC分类号： C12Q1/34 ,  C12Q1/68 ,  G01N33/573

CPC分类号： G01N33/689 ,  C12N9/80 ,  C12Q1/6881 ,  C12Q2600/158

摘要： The present invention relates to a method of predicting the likelihood of embryo or oocyte survival. This method involves providing a sample of an embryo, an oocyte, or surrounding liquid or cells, and screening the sample for ceramidase expression or activity. The ceramidase expression or activity obtained through said screening is then correlated to a prediction of the likelihood of embryo or oocyte survival. Also disclosed is a kit.

摘要翻译： 本发明涉及预测胚胎或卵母细胞存活的可能性的方法。 该方法包括提供胚胎，卵母细胞或周围液体或细胞的样品，并筛选样品用于神经酰胺酶表达或活性。 然后通过所述筛选获得的神经酰胺酶表达或活性与胚胎或卵母细胞存活的可能性的预测相关。 还公开了一种工具箱。

公开(公告)号：US06790627B2

公开(公告)日：2004-09-14

申请号：US09928872

申请日：2001-08-13

申请人： Richard Kolesnick ,  Edward H. Schuchman

发明人： Richard Kolesnick ,  Edward H. Schuchman

IPC分类号： G01N3353

CPC分类号： G01N33/502 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  C12N9/16 ,  C12N15/8509 ,  G01N33/5008 ,  G01N33/5073 ,  G01N2510/00

摘要： The present invention relates, first, to methods for the modulation of acid sphingomyelinase (ASM)-related processes, including apoptosis. Such apoptosis can include, but is not limited to, environmental stress-induced apoptosis such as, for example, ionizing radiation and/or chemotherapeutic agent-induced apoptosis. Apoptosis can be characterized by a cellular morphology comprising cellular condensation, nuclear condensation or zeiosis. The present invention further relates to methods for the identification of compounds which modulate (i.e., either increase or decrease) sensitivity to ASM-related processes, including apoptosis.

摘要翻译： 本发明首先涉及调节酸性鞘磷脂酶（ASM）相关过程（包括细胞凋亡）的方法。 这种凋亡可以包括但不限于环境胁迫诱导的凋亡，例如电离辐射和/或化学治疗剂诱导的凋亡。 凋亡可以通过细胞形态来表征，包括细胞凝结，核缩合或大肠杆菌。 本发明还涉及用于鉴定调节（即，增加或减少）对ASM相关过程（包括凋亡）的敏感性的化合物的方法。

公开(公告)号：US06274309B1

公开(公告)日：2001-08-14

申请号：US08687707

申请日：1996-07-26

申请人： Richard Kolesnick ,  Edward H. Schuchman

发明人： Richard Kolesnick ,  Edward H. Schuchman

IPC分类号： C12Q102

CPC分类号： G01N33/502 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  C12N9/16 ,  C12N15/8509 ,  G01N33/5008 ,  G01N33/5073 ,  G01N2510/00

摘要： The present invention relates, first, to methods and compositions for the modulation of acid sphingomyelinase (ASM)-related processes, including apoptosis. Such apoptosis can include, but is not limited to, environmental stress-induced apoptosis such as, for example, ionizing radiation and/or chemotherapeutic agent-induced apoptosis. Apoptosis can be characterized by a cellular morphology comprising cellular condensation, nuclear condensation or zeiosis. The present invention further relates to methods for the identification of compounds which modulate (i.e., either increase or decrease) sensitivity to ASM-related processes, including apoptosis.

摘要翻译： 本发明首先涉及用于调节酸性鞘磷脂酶（ASM）相关过程（包括凋亡）的方法和组合物。 这种凋亡可以包括但不限于环境应激诱导的凋亡，例如电离辐射和/或化学治疗剂诱导的凋亡。 凋亡可以通过细胞形态来表征，包括细胞凝结，核缩合或大肠杆菌。 本发明还涉及用于鉴定调节（即，增加或减少）对ASM相关过程（包括凋亡）的敏感性的化合物的方法。