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    Seed plants exhibiting inducible early reproductive development and methods of making same
    11.
    发明授权
    Seed plants exhibiting inducible early reproductive development and methods of making same 失效
    表现出诱导性早期生殖发育的种子植物及其制备方法

    公开(公告)号：US06355863B1

    公开(公告)日：2002-03-12

    申请号：US09398326

    申请日：1999-09-16

    申请人： Martin F. Yanofsky

    发明人： Martin F. Yanofsky

    IPC分类号： A01H500

    CPC分类号： C07K14/415 ,  C12N15/8261 ,  C12N15/827 ,  Y02A40/146

    摘要： The present invention provides a recombinant nucleic acid molecule containing an inducible regulatory element operably linked to a nucleic acid molecule encoding a floral meristem identity gene product such as APETALA1 (AP1), CAULIFLOWER (CAL) or LEAFY (LFY). The invention also provides a transgenic seed plant, such as an angiosperm or gymnosperm, that contains a recombinant nucleic acid molecule of the invention. The invention further provides methods, such as methods of converting shoot meristem to floral meristem in a transgenic angiosperm and methods of promoting early reproductive development in a seed plant, which use a recombinant nucleic acid molecule of the invention. In addition, the invention provides a nucleic acid molecule encoding a chimeric protein, which contains a nucleic acid molecule encoding a floral meristem identity gene product linked in frame to a nucleic acid molecule encoding a ligand binding domain. The invention also provides a transgenic seed plant, such as an angiosperm or gymnosperm, that contains a nucleic acid molecule encoding a chimeric protein of the invention. Also provided herein are methods, such as methods of converting shoot meristem to floral meristem in a transgenic angiosperm and methods of promoting early reproductive development in a seed plant, which use a nucleic acid molecule encoding a chimeric protein of the invention.

    摘要翻译： 本发明提供了含有可操作地连接到编码花分生组织同一性基因产物如APETALA1（AP1），CAULIFLOWER（CAL）或LEAFY（LFY））的核酸分子的诱导型调控元件的重组核酸分子。 本发明还提供了含有本发明的重组核酸分子的转基因种子植物，例如被子植物或裸子植物。 本发明还提供了使用本发明的重组核酸分子的方法，例如转基因被子植物中的枝条分生组织转化为花分生组织的方法和促进种子植物中早期繁殖发育的方法。 此外，本发明提供编码嵌合蛋白的核酸分子，其含有编码与编码配体结合结构域的核酸分子框架连接的花分生组织同一性基因产物的核酸分子。 本发明还提供了含有编码本发明的嵌合蛋白质的核酸分子的转基因种子植物，例如被子植物或裸子植物。 本文还提供了诸如在转基因被子植物中转化枝条分生组织至花分生组织的方法以及使用编码本发明的嵌合蛋白质的核酸分子在种子植物中促进早期繁殖发育的方法。

    Cauliflower floral meristem identity genes and methods of using same
    12.
    发明授权
    Cauliflower floral meristem identity genes and methods of using same 失效
    花椰菜花分生组织识别基因及使用方法

    公开(公告)号：US6127123A

    公开(公告)日：2000-10-03

    申请号：US149976

    申请日：1998-09-09

    申请人： Martin F. Yanofsky

    发明人： Martin F. Yanofsky

    IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6895 ,  C07K14/415 ,  C12N15/827 ,  C12Q2600/13 ,  C12Q2600/156

    摘要： The present invention provides a nucleic acid molecule encoding a CAULIFLOWER (CAL) gene product such as a nucleic acid molecule encoding Arabidopsis thaliana CAL and a nucleic acid molecule encoding Brassica oleracea CAL (BoCAL). The invention also provides a nucleic acid molecule encoding a truncated CAL gene product such as a nucleic acid molecule encoding Brassica oleracea var. botrytis CAL (BobCAL). The invention also provides a nucleic acid containing the Arabidopsis thaliana CAL gene, a nucleic acid molecule containing the Brassica oleracea CAL gene and a nucleic acid molecule containing the Brassica oleracea var. botrytis CAL gene. The invention further provides a kit for converting shoot meristem to floral meristem and a kit for promoting early flowering in an angiosperm. The invention provides a CAL polypeptide and an antibody that specifically binds CAL polypeptide. In addition, the invention provides the truncated BobCAL polypeptide and an antibody that specifically binds truncated BobCAL polypeptide. The invention further provides a method of identifying a Brassica having a modified CAL allele by detecting a polymorphism associated with a CAL locus, where the CAL locus comprises a modified CAL allele that does not encode an active CAL gene product.

    摘要翻译： 本发明提供了编码CAULIFLOWER（CAL）基因产物的核酸分子，例如编码拟南芥CAL的核酸分子和编码甘蓝CARC（BoCAL）的核酸分子。 本发明还提供编码截短的CAL基因产物的核酸分子，例如编码甘蓝型油菜的核酸分子。 botrytis CAL（BobCAL）。 本发明还提供了含有拟南芥CAL基因的核酸，含有甘蓝型甘蓝CAL基因的核酸分子和含有甘蓝型甘蓝变种的核酸分子。 Botrytis CAL基因。 本发明还提供了用于将枝条分生组织转化为花分生组织的试剂盒和用于促进被子植物早期开花的试剂盒。 本发明提供了CAL多肽和特异性结合CAL多肽的抗体。 此外，本发明提供截短的BobCAL多肽和特异性结合截短的BobCAL多肽的抗体。 本发明还提供了通过检测与CAL基因座相关的多态性鉴定具有修饰的CAL等位基因的芸苔属的方法，其中所述CAL基因座包含不编码活性CAL基因产物的修饰的CAL等位基因。

    Maize and cauliflower apetalai gene products and nucleic acid molecules encoding same
    13.
    发明授权
    Maize and cauliflower apetalai gene products and nucleic acid molecules encoding same 失效
    玉米和花椰菜apetalai基因产物和核酸分子编码相同

    公开(公告)号：US6025483A

    公开(公告)日：2000-02-15

    申请号：US655227

    申请日：1996-06-05

    申请人： Martin F. Yanofsky

    发明人： Martin F. Yanofsky

    IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82

    CPC分类号： C12N15/827 ,  C07K14/415

    摘要： The present invention provides a substantially purified nucleic acid molecule encoding a Brassica oleracea APETALA1 (AP1) gene product, Brassica oleracea var. botrytis AP1 gene product or a Zea mays AP1 gene product. The invention also provides a substantially purified Brassica oleracea AP1 gene product, a substantially purified Brassica oleracea var. botrytis AP1 gene product or a substantially purified Zea mays AP1 gene product in addition to an antibody that specifically binds such an AP1 gene product. In addtion, the invention provides an expression vector containing a nucleic acid molecule encoding a floral meristem identity gene product operably linked to a heterologous regulatory element. Kits for converting shoot meristem to floral meristem in an angiosperm and kits for promoting early reproductive development in a seed plant also are provided.

    摘要翻译： 本发明提供了一种基本上纯化的核酸分子，其编码甘蓝型APETALA1（AP1）基因产物，甘蓝油菜（Brassica oleracea var。 Botrytis AP1基因产物或Zea mays AP1基因产物。 本发明还提供了一种基本上纯化的甘蓝AP1基因产物，一种基本上纯化的甘蓝型甘蓝变种。 除了特异性结合这样的AP1基因产物的抗体之外，还有一种基因纯化的酵母属AP1基因产物或基本纯化的玉米AP1基因产物。 另外，本发明提供含有编码与异源调节元件可操作连接的花分生组织同一性基因产物的核酸分子的表达载体。 还提供了用于将枝条分生组织转化为被子植物中的花分生组织和用于促进种子植物中的早期生殖发育的试剂盒。

    Seed plants exhibiting inducible early reproductive development and methods of making same
    14.
    发明授权
    Seed plants exhibiting inducible early reproductive development and methods of making same 失效

    公开(公告)号：US6002069A

    公开(公告)日：1999-12-14

    申请号：US659188

    申请日：1996-06-05

    申请人： Martin F. Yanofsky

    发明人： Martin F. Yanofsky

    IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00

    CPC分类号： C07K14/415 ,  C12N15/8261 ,  C12N15/827

    摘要： The present invention provides a recombinant nucleic acid molecule containing an inducible regulatory element operably linked to a nucleic acid molecule encoding a floral meristem identity gene product such as APETALA1 (AP1), CAULIFLOWER (CAL) or LEAFY (LFY). The invention also provides a transgenic seed plant, such as an angiosperm or gymnosperm, that contains a recombinant nucleic acid molecule of the invention. The invention further provides methods, such as methods of converting shoot meristem to floral meristem in a transgenic angiosperm and methods of promoting early reproductive development in a seed plant, which use a recombinant nucleic acid molecule of the invention. In addition, the invention provides a nucleic acid molecule encoding a chimeric protein, which contains a nucleic acid molecule encoding a floral meristem identity gene product linked in frame to a nucleic acid molecule encoding a ligand binding domain. The invention also provides a transgenic seed plant, such as an angiosperm or gymnosperm, that contains a nucleic acid molecule encoding a chimeric protein of the invention. Also provided herein are methods, such as methods of converting shoot meristem to floral meristem in a transgenic angiosperm and methods of promoting early reproductive development in a seed plant, which use a nucleic acid molecule encoding a chimeric protein of the invention.