公开(公告)号：US20150099650A1

公开(公告)日：2015-04-09

申请号：US14047233

申请日：2013-10-07

Applicant: General Electric Company

Inventor： Anup Sood ,  Arunkumar Natarajan ,  Michael James Rishel ,  Michael John Gerdes

IPC: C12Q1/68

CPC classification number: C12Q1/6825 ,  C12Q2523/319 ,  G01N33/53 ,  G01N33/533 ,  G01N33/542 ,  G01N33/6854

Abstract: Disclosed are high throughput methods of probing multiple targets in a biological sample where the recurrent time-consuming antibody incubation steps and individual signal modification and activation steps are replaced by simultaneous hybridization of biomarkers and sequential detection by combining signal removal and activation into a single step. Also disclosed are images obtained by such methods.

Abstract translation: 公开了探测生物样品中多个靶标的高通量方法，其中复发耗时的抗体孵育步骤和单独的信号修饰和激活步骤被生物标志物的同时杂交和通过将信号去除和活化组合到单个步骤中的顺序检测来代替。 还公开了通过这种方法获得的图像。