公开(公告)号：US20240321393A1

公开(公告)日：2024-09-26

申请号：US18580053

申请日：2022-07-27

申请人： The Regents of the University of California

发明人： Roy WOLLMAN ,  Zachary HEMMINGER

IPC分类号： G16B25/10 ,  C12Q1/6825 ,  G01N1/30 ,  G01N21/64 ,  G01N33/53 ,  G01N33/58 ,  G01N33/68 ,  G16H40/20

CPC分类号： G16B25/10 ,  C12Q1/6825 ,  G01N1/30 ,  G01N21/6456 ,  G01N33/53 ,  G01N33/58 ,  G01N33/6803 ,  G16H40/20

摘要： Methods are described for cell-type mapping utilizing direct measurement of low-dimensional representation of single-cell transcriptomics with a supervised machine learning algorithm to spatially map cell types, bypassing the need to measure expression of single genes. Such methods are useful to identify locations of cell types in biological specimens for purposes including tissue based diagnostics.

公开(公告)号：US20240309432A1

公开(公告)日：2024-09-19

申请号：US18668982

申请日：2024-05-20

申请人： Bruker Spatial Biology, Inc.

发明人： Rustem KHAFIZOV ,  Dwayne L. DUNAWAY ,  Mark GREGORY

IPC分类号： C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6834

CPC分类号： C12Q1/6825 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6834

摘要： The present disclosure relates to chemical compositions, kits, and apparatuses and methods for using these compositions, kits and apparatuses in various assays.

公开(公告)号：US12071664B2

公开(公告)日：2024-08-27

申请号：US17939760

申请日：2022-09-07

申请人： Pacific Biosciences of California, Inc.

发明人： Adrian Fehr ,  Nathaniel Joseph McCaffrey ,  Stephen Turner

IPC分类号： C12Q1/6874 ,  B01L3/00 ,  B82Y20/00 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/03 ,  G01N21/05 ,  G01N21/64 ,  G01N21/75 ,  G01N21/77 ,  G01N33/543 ,  G02B6/122

CPC分类号： C12Q1/6874 ,  B01L3/502707 ,  B01L3/502715 ,  B82Y20/00 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N21/03 ,  G01N21/0303 ,  G01N21/05 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6454 ,  G01N21/6456 ,  G01N21/648 ,  G01N21/75 ,  G01N21/77 ,  G01N33/54373 ,  G02B6/1226 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/168 ,  G01N2021/0346 ,  G01N2021/6434 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/757 ,  G01N2021/7786 ,  G01N2201/067 ,  G01N2201/068 ,  G01N2201/08 ,  Y10T436/143333

摘要： Optics collection and detection systems are provided for measuring optical signals from an array of optical sources over time. Methods of using the optics collection and detection systems are also described.

公开(公告)号：US20240271187A1

公开(公告)日：2024-08-15

申请号：US18022098

申请日：2021-08-18

申请人： Sherlock Biosciences, Inc.

发明人： William Jeremy Blake ,  Paul David Carlson ,  Elizabeth Anne Phillips ,  Michael Menglong Liu ,  Carl Wayne Brown, III ,  Mary Katherine Wilson ,  Brendan John Manning

IPC分类号： C12Q1/6825 ,  C12Q1/6897

CPC分类号： C12Q1/6825 ,  C12Q1/6897

摘要： The present disclosure provides compositions and methods that permit detection of nucleic acids in samples (e.g., biological and/or environmental samples). The present disclosure describes “split reporter” sensor system technologies.

公开(公告)号：US12053780B2

公开(公告)日：2024-08-06

申请号：US17470615

申请日：2021-09-09

申请人： CUE HEALTH INC.

发明人： Ayub Khattak ,  Clinton Sever ,  Paul Nelson ,  Ryan Cooper ,  Thomas Congdon ,  Justin Demartino ,  Raphael Shapiro ,  Mark Duncan

IPC分类号： B01L3/00 ,  A61B10/00 ,  A61B10/02 ,  B65D83/00 ,  B65D83/04 ,  B65D83/08 ,  G01N27/327 ,  G01N33/543 ,  G01N35/00 ,  G01N35/10 ,  A61B5/157 ,  C12Q1/6825

CPC分类号： B01L3/508 ,  A61B10/0045 ,  A61B10/02 ,  B01L3/5023 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/5029 ,  B01L3/523 ,  B65D83/005 ,  B65D83/04 ,  B65D83/0817 ,  B65D83/0858 ,  G01N27/3273 ,  G01N33/54366 ,  G01N35/0098 ,  G01N35/10 ,  A61B5/157 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/023 ,  B01L2300/047 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0672 ,  B01L2300/069 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/1805 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0481 ,  B01L2400/0677 ,  C12Q1/6825

摘要： Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

公开(公告)号：US20240240239A1

公开(公告)日：2024-07-18

申请号：US18560358

申请日：2022-03-22

申请人： XIAMEN SEERNA BIOSCIENCE CO., LTD.

发明人： Ling LIU ,  Lihua HUANG ,  Rongqin KE

IPC分类号： C12Q1/6825 ,  C12Q1/25 ,  C12Q1/48 ,  C12Q1/6844 ,  C12Q1/6876

CPC分类号： C12Q1/6825 ,  C12Q1/25 ,  C12Q1/485 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N2333/9015 ,  G01N2333/9126

摘要： The present invention relates to a method for testing the presence or level of one or more target nucleic acids in a sample, and further relates to a probe set and a kit comprising one or more probe sets.

公开(公告)号：US20240233876A1

公开(公告)日：2024-07-11

申请号：US18402438

申请日：2024-01-02

申请人： The Board of Trustees of the University of Illinois

发明人： Jean-Pierre Leburton ,  Olgica Milenkovic ,  Rajat Chakraborty

IPC分类号： G16B50/30 ,  C12Q1/6825 ,  G01N33/487

CPC分类号： G16B50/30 ,  C12Q1/6825 ,  G01N33/48721

摘要： Systems and methods are provided for encoding information as the pattern of the lengths of RNA tails (or other tail biomolecules, e.g., polypeptides) attached to a dsDNA (or other backbone biomolecule) and the relative locations of attachment thereto. This information can be quickly and accurately read out by passing such information-encoding payload biomolecules through a nanopore and detecting the pattern of conductance of the pore (e.g., the ionic conductance through the pore and/or the transverse conductance of the membrane bearing the pore) as the payload passes therethrough. The conductance can be processed by subtracting a time-varying correction factor therefrom that represents the expected conductance for a ‘tail-free’ backbone as it transits through the pore, by applying a time-varying velocity factor that is dependent on the number of tails present in the pore over time to normalize individual tail dwell times, and/or other processing steps.

公开(公告)号：US20240229119A1

公开(公告)日：2024-07-11

申请号：US18617874

申请日：2024-03-27

申请人： Chang Gung University

发明人： Min-Hsien WU ,  Chih-Yu CHEN

IPC分类号： C12Q1/6844 ,  A61K38/48 ,  B01L7/00 ,  C12Q1/6825 ,  G01N33/543

CPC分类号： C12Q1/6844 ,  A61K38/4893 ,  B01L7/52 ,  C12Q1/6825 ,  G01N33/54388 ,  B01L2300/0663 ,  B01L2300/1805 ,  B01L2300/1894

摘要： The present invention relates to a nucleic acid amplification method and device, and a nucleic acid detection method and device. The nucleic acid amplification device comprises a reaction unit, an energy excitation unit, an operation unit, and an auxiliary cooling unit; the reaction unit comprises a target analyte to be detected, one or more solid-phase carriers, and a nucleic acid amplification reaction solution; each solid-phase carrier has a functionalized specific surface ligand to provide purification, separation, and nucleic acid amplification reaction of a target analyte to be detected; and energy output and an enabling/disabling time sequence of the energy excitation unit are controlled to generate a reaction temperature (thermal) cycle, so that an in-situ environment is formed around each solid-phase carrier during the cycle, and in-situ nucleic acid amplification reaction is performed in the in-situ environment to generate amplicons.

公开(公告)号：US20240229110A1

公开(公告)日：2024-07-11

申请号：US18544734

申请日：2023-12-19

申请人： MESO SCALE TECHNOLOGIES, LLC.

发明人： John KENTEN ,  Galina NIKOLENKO ,  John Robert PETTERSSON

IPC分类号： C12Q1/6825 ,  C12Q1/682

CPC分类号： C12Q1/6825 ,  C12Q1/682 ,  C12Q1/6806

摘要： The invention provides novel components for conducting assays, e.g., sandwich immunoassays, methods utilizing the novel components, and compositions and kits comprising the novel components.

公开(公告)号：US20240218441A1

公开(公告)日：2024-07-04

申请号：US18397221

申请日：2023-12-27

申请人： IMEC VZW

发明人： Koen Martens ,  Nick Van Helleputte ,  Pol Van Dorpe ,  Sybren Santermans

IPC分类号： C12Q1/6869 ,  C12Q1/6825

CPC分类号： C12Q1/6869 ,  C12Q1/6825

摘要： The present disclosure relates to single-peptide sequencing. A method for sequencing peptides is provided that includes attaching a base end of a peptide to a channel region surface of a biosensor field effect transistor (bio-FET) and obtaining a first measurement of a parameter of the bio-FET, the parameter depending on the bio-FET's threshold voltage. The method further includes providing first molecular probes into the electrolyte gate, each first molecular probe being linked to a respective charge tag, and obtaining a second measurement of the parameter. Then, the method includes determining whether a shift of the parameter occurred between the first and second measurements, which indicates that a first molecular probe attached to an amino acid at a free end of the peptide. If the shift of the parameter occurred, the method includes determining a type of the amino acid based on a type of the first molecular probe.