公开(公告)号：US20110229776A1

公开(公告)日：2011-09-22

申请号：US13130475

申请日：2009-11-24

申请人： Hideyuki Kumita ,  Takaaki Nakagawa ,  Hideki Sakai ,  Masaya Kakuta ,  Yuichi Tokita

发明人： Hideyuki Kumita ,  Takaaki Nakagawa ,  Hideki Sakai ,  Masaya Kakuta ,  Yuichi Tokita

IPC分类号： H01M4/88 ,  H01M8/00 ,  H01M8/16 ,  H01M4/90 ,  C12N9/00 ,  C12N9/02

CPC分类号： H01M8/16 ,  H01M4/8825 ,  H01M4/8882 ,  H01M4/90 ,  Y02E60/527 ,  Y02P70/56

摘要： Provided are an enzyme immobilizing method, a fuel cell and an electrode for the fuel cell which employ the enzyme immobilizing method, and a method for manufacturing the fuel cell and the electrode. The enzyme immobilizing method prevents reduction in enzyme activity when the enzyme is immobilized on the electrode, so as to make it possible to obtain a high catalyst current value. In the method for immobilizing an enzyme on the electrode used in the fuel cell, an enzyme variant with at least one amino acid residue being deleted, substituted, added, or inserted in a wild-type amino acid sequences is used as the enzyme, and the enzyme variant increases in activity through heat treatment. The immobilization is performed within a temperature range which makes it possible to increase the activity of the enzyme variant.

摘要翻译： 提供了酶固定化方法，燃料电池和使用酶固定化方法的燃料电池用电极，以及制造燃料电池和电极的方法。 当酶固定在电极上时，酶固定化方法防止酶活性的降低，从而可以获得高的催化剂电流值。 在将酶固定在燃料电池中使用的电极上的方法中，使用缺失，取代，添加或插入到野生型氨基酸序列中的至少一个氨基酸残基的酶变体作为酶， 酶变体通过热处理增加活性。 在可以增加酶变体的活性的温度范围内进行固定化。

公开(公告)号：US20110065008A1

公开(公告)日：2011-03-17

申请号：US12990919

申请日：2009-04-23

申请人： Takaaki Nakagawa ,  Hideyuki Kumita ,  Yoshio Goto ,  Hideki Sakai ,  Masaya Kakuta

发明人： Takaaki Nakagawa ,  Hideyuki Kumita ,  Yoshio Goto ,  Hideki Sakai ,  Masaya Kakuta

IPC分类号： H01M8/16 ,  C12N11/00

CPC分类号： H01M8/16 ,  C12N9/0004 ,  H01M4/9008 ,  Y02E60/527

摘要： Provided is an enzyme electrode in which oxidation-reduction reactions proceed with an enzyme acting as a catalyst, and the enzyme is modified to increase affinity and/or reaction rate with a reaction substrate or an electron transfer mediator by adding or inserting at least one codon encoding a particular amino acid residue to or into a base sequence encoding the enzyme, and is immobilized. Since the oxidation-reduction reactions on the electrode proceed highly efficiently, the enzyme electrode can cause to increase the obtained output of electric energy and thus can be suitably used in all types of fuel cells, biosensors, and electronic apparatuses.

摘要翻译： 提供了一种酶电极，其中用作催化剂的酶进行氧化还原反应，并且通过添加或插入至少一个密码子来修饰酶以增加与反应底物或电子转移介体的亲和力和/或反应速率 将特定氨基酸残基编码到编码该酶的碱基序列中或被固定化，并被固定化。 由于电极上的氧化还原反应高效地进行，所以酶电极可以增加获得的电能输出，因此可以适用于所有类型的燃料电池，生物传感器和电子设备。

公开(公告)号：US20130065139A1

公开(公告)日：2013-03-14

申请号：US13699067

申请日：2011-06-02

申请人： Takaaki Nakagawa ,  Tsunetoshi Samukawa ,  Hiroki Mita ,  Taiki Sugiyama ,  Ryuhei Matsumoto ,  Hideki Sakai

发明人： Takaaki Nakagawa ,  Tsunetoshi Samukawa ,  Hiroki Mita ,  Taiki Sugiyama ,  Ryuhei Matsumoto ,  Hideki Sakai

IPC分类号： H01M8/16

CPC分类号： H01M4/9008 ,  H01M8/0289 ,  H01M8/04197 ,  H01M8/1067 ,  H01M8/16 ,  Y02E60/527

摘要： A fuel cell that can have a higher battery capacity without degradation of cathode characteristics is provided.In a biofuel cell that includes one or more battery cell units (1) in which an oxidoreductase exists on the surface of an anode (2) and/or a cathode (3), and the cathode (3) is in contact with both a liquid phase and a gas phase, a selective transmission film (6) that restrains permeation of at least the fuel component is provided between an anode solution unit (4) provided around the anode (2) and a cathode solution unit (5) provided around the cathode (3). The fuel component concentration in the solution in contact with the anode (2) is higher than that in the solution in contact with the cathode (3).

摘要翻译： 提供了可以具有更高电池容量而不降低阴极特性的燃料电池。 在包括其中在阳极（2）和/或阴极（3）的表面上存在氧化还原酶的一个或多个电池单元（1）的生物燃料电池中，并且阴极（3）与两者 液相和气相，在设置在阳极（2）周围的阳极溶液单元（4）和设置在阳极（2）周围的阴极溶液单元（5）之间设置抑制至少燃料成分渗透的选择透射膜（6） 阴极（3）。 与阳极（2）接触的溶液中的燃料成分浓度高于与阴极（3）接触的溶液中的燃料成分浓度。

公开(公告)号：US20140065494A1

公开(公告)日：2014-03-06

申请号：US13982099

申请日：2012-01-24

申请人： Shuji Fujita ,  Hiroki Mita ,  Shun Yamanoi ,  Hideki Sakai ,  Yuichi Tokita ,  Seiichiro Tabata ,  Tsunetoshi Samukawa ,  Takaaki Nakagawa

发明人： Shuji Fujita ,  Hiroki Mita ,  Shun Yamanoi ,  Hideki Sakai ,  Yuichi Tokita ,  Seiichiro Tabata ,  Tsunetoshi Samukawa ,  Takaaki Nakagawa

IPC分类号： H01M4/90 ,  C25B11/04 ,  G01N27/327

CPC分类号： H01M4/9008 ,  C25B11/0447 ,  G01N27/327 ,  H01M4/9083 ,  H01M8/16 ,  Y02E60/527 ,  Y02P70/56

摘要： Provided is a fuel cell capable of preventing elution of nicotinamide adenine dinucleotide and/or a derivative thereof immobilized on an electrode, and capable of preventing performance degradation due to elution, and a method for manufacturing the fuel cell.A biofuel cell having a structure in which a positive electrode and a negative electrode face each other via a proton conductor, the biofuel cell configured so that an enzyme is used to extract electrons from a fuel, wherein the negative electrode is configured from an electrode including carbon and/or an inorganic compound having pores with a size of 2 nm or more and 100 nm or less on the surface, nicotinamide adenine dinucleotide and/or a derivative thereof being immobilized on the carbon and/or the inorganic compound. A carbon particle, a carbon sheet, or carbon fiber is used as the carbon.

摘要翻译： 提供一种燃料电池，其能够防止固定在电极上的烟酰胺腺嘌呤二核苷酸和/或其衍生物的洗脱，并且能够防止由于洗脱引起的性能劣化，以及燃料电池的制造方法。 一种生物燃料电池，其具有通过质子导体将正极和负极面对的结构，所述生物燃料电池被配置为使得酶用于从燃料中提取电子，其中所述负极由电极构成，所述电极包括 碳和/或具有尺寸为2nm以上且100nm以下的孔的无机化合物，烟酰胺腺嘌呤二核苷酸和/或其衍生物固定在碳和/或无机化合物上。 使用碳粒子，碳片或碳纤维作为碳。

公开(公告)号：US08841036B2

公开(公告)日：2014-09-23

申请号：US13087470

申请日：2011-04-15

申请人： Shuji Fujita ,  Takaaki Nakagawa ,  Ryuhei Matsumoto ,  Hideyuki Kumita ,  Daisuke Yamaguchi

发明人： Shuji Fujita ,  Takaaki Nakagawa ,  Ryuhei Matsumoto ,  Hideyuki Kumita ,  Daisuke Yamaguchi

IPC分类号： H01M8/04 ,  H01M8/24 ,  H01M2/38 ,  H01M2/40 ,  H01M8/16

CPC分类号： H01M8/16 ,  Y02E60/527

摘要： Disclosed herein is a flexible fuel cell including, one or a plurality of cell sections, and a sealing sheet covering the cell section or sections, wherein the cell section has, at least, a pair of electrode sheets which form an anode and a cathode and at least one of which is accompanied by an oxidoreductase present at a surface thereof, a separator which is disposed between the electrode sheets and which has a proton-permeable membrane, a pair of current collectors which are electrically conductively connected respectively to the electrode sheets with a conductive adhesive, and a fuel reservoir section which is provided at such a position as to make contact with the anode at least and in which a fuel solution containing a fuel component is reserved.

摘要翻译： 本文公开了一种柔性燃料电池，其包括一个或多个电池部分，以及覆盖电池部分的密封片，其中电池部分至少具有一对形成阳极和阴极的电极片， 其中至少一个伴随着存在于其表面的氧化还原酶，隔离物设置在电极片之间并具有质子渗透膜，一对集电体分别与电极片电连接， 导电粘合剂和燃料储存部分，其设置在至少与阳极接触的位置处，并且其中保留了含有燃料部件的燃料溶液。

公开(公告)号：US20110287328A1

公开(公告)日：2011-11-24

申请号：US13087470

申请日：2011-04-15

申请人： Shuji Fujita ,  Takaaki Nakagawa ,  Ryuhei Matsumoto ,  Hideyuki Kumita ,  Daisuke Yamaguchi

发明人： Shuji Fujita ,  Takaaki Nakagawa ,  Ryuhei Matsumoto ,  Hideyuki Kumita ,  Daisuke Yamaguchi

IPC分类号： H01M8/16

CPC分类号： H01M8/16 ,  Y02E60/527

摘要： Disclosed herein is a flexible fuel cell including, one or a plurality of cell sections, and a sealing sheet covering the cell section or sections, wherein the cell section has, at least, a pair of electrode sheets which form an anode and a cathode and at least one of which is accompanied by an oxidoreductase present at a surface thereof, a separator which is disposed between the electrode sheets and which has a proton-permeable membrane, a pair of current collectors which are electrically conductively connected respectively to the electrode sheets with a conductive adhesive, and a fuel reservoir section which is provided at such a position as to make contact with the anode at least and in which a fuel solution containing a fuel component is reserved.

摘要翻译： 本文公开了一种柔性燃料电池，其包括一个或多个电池部分，以及覆盖电池部分的密封片，其中电池部分至少具有一对形成阳极和阴极的电极片， 其中至少一个伴随着存在于其表面的氧化还原酶，隔离物设置在电极片之间并具有质子渗透膜，一对集电体分别与电极片电连接， 导电粘合剂和燃料储存部分，其设置在至少与阳极接触的位置处，并且其中保留了含有燃料部件的燃料溶液。

公开(公告)号：US20110171541A1

公开(公告)日：2011-07-14

申请号：US12672582

申请日：2008-07-31

申请人： Takaaki Nakagawa ,  Hideki Sakai ,  Hideyuki Kumita ,  Masaya Kakuta

发明人： Takaaki Nakagawa ,  Hideki Sakai ,  Hideyuki Kumita ,  Masaya Kakuta

IPC分类号： H01M8/16

CPC分类号： H01M8/16 ,  C12N11/14 ,  H01M4/8605 ,  H01M2004/8689 ,  Y02E60/527

摘要： Provided is a fuel cell having a structure in which a cathode and an anode face each other with a proton conductor therebetween. In this fuel cell, an oxygen reductase or the like is immobilized on at least the cathode, and the cathode is composed of a material having pores therein such as porous carbon. In this fuel cell, the volume of water contained in the cathode is controlled to be 70% or less of the volume of the pores of the cathode, whereby a high current value can be stably obtained through optimization of the amount of moisture contained in the cathode when an enzyme is immobilized on at least the cathode. Also provided is a method for operating the fuel cell.

摘要翻译： 提供一种燃料电池，其具有阴极和阳极彼此面对的质子导体的结构。 在该燃料电池中，氧还原酶等固定在至少阴极上，阴极由其中具有孔的材料如多孔碳构成。 在该燃料电池中，将阴极中含有的水的体积控制为阴极的孔的体积的70％以下，由此通过优化包含在阴极中的水分量，能够稳定地获得高电流值 当酶至少固定在阴极上时是阴极。 还提供了一种操作燃料电池的方法。

公开(公告)号：US20130130130A1

公开(公告)日：2013-05-23

申请号：US13699061

申请日：2011-04-25

申请人： Daisuke Yamaguchi ,  Tsunetoshi Samukawa ,  Shuji Fujita ,  Hiroki Mita ,  Takaaki Nakagawa ,  Yuichi Tokita

发明人： Daisuke Yamaguchi ,  Tsunetoshi Samukawa ,  Shuji Fujita ,  Hiroki Mita ,  Takaaki Nakagawa ,  Yuichi Tokita

IPC分类号： H01M8/04

CPC分类号： H01M8/04201 ,  H01M8/04082 ,  H01M8/16 ,  H01M8/2455 ,  Y02E60/527

摘要： The valve control unit of a fuel leakage preventing structure of the present invention includes first and second electrodes. The first electrode is attached to a first member that is movable. When a pressing force is applied to the first member to move, the first and second electrodes are brought into contact with each other, to cause conduction. The first and second electrodes are connected to a control device, and the control device opens a control valve when the first and second electrodes are put into a conducting state. As the control valve is opened and closed by a pressing force in this manner, a fuel solution can be easily supplied.

摘要翻译： 本发明的燃料泄漏防止结构的阀门控制单元包括第一和第二电极。 第一电极附接到可移动的第一构件。 当对第一构件施加按压力以移动时，第一和第二电极彼此接触以导致传导。 第一和第二电极连接到控制装置，并且当第一和第二电极进入导通状态时，控制装置打开控制阀。 由于通过这种方式通过按压力打开和关闭控制阀，所以可以容易地供应燃料溶液。

公开(公告)号：US08911909B2

公开(公告)日：2014-12-16

申请号：US13699061

申请日：2011-04-25

申请人： Daisuke Yamaguchi ,  Tsunetoshi Samukawa ,  Shuji Fujita ,  Hiroki Mita ,  Takaaki Nakagawa ,  Yuichi Tokita

发明人： Daisuke Yamaguchi ,  Tsunetoshi Samukawa ,  Shuji Fujita ,  Hiroki Mita ,  Takaaki Nakagawa ,  Yuichi Tokita

IPC分类号： H01M8/16 ,  H01M8/04 ,  H01M8/24

CPC分类号： H01M8/04201 ,  H01M8/04082 ,  H01M8/16 ,  H01M8/2455 ,  Y02E60/527

摘要： The valve control unit of a fuel leakage preventing structure of the present invention includes first and second electrodes. The first electrode is attached to a first member that is movable. When a pressing force is applied to the first member to move, the first and second electrodes are brought into contact with each other, to cause conduction. The first and second electrodes are connected to a control device, and the control device opens a control valve when the first and second electrodes are put into a conducting state. As the control valve is opened and closed by a pressing force in this manner, a fuel solution can be easily supplied.

摘要翻译： 本发明的燃料泄漏防止结构的阀门控制单元包括第一和第二电极。 第一电极附接到可移动的第一构件。 当对第一构件施加按压力以移动时，第一和第二电极彼此接触以导致传导。 第一和第二电极连接到控制装置，并且当第一和第二电极进入导通状态时，控制装置打开控制阀。 由于通过这种方式通过按压力打开和关闭控制阀，所以可以容易地供应燃料溶液。

公开(公告)号：US08808928B2

公开(公告)日：2014-08-19

申请号：US12672582

申请日：2008-07-31

申请人： Takaaki Nakagawa ,  Hideki Sakai ,  Hideyuki Kumita ,  Masaya Kakuta

发明人： Takaaki Nakagawa ,  Hideki Sakai ,  Hideyuki Kumita ,  Masaya Kakuta

IPC分类号： H01M8/16 ,  H01M8/04 ,  H01M4/60

CPC分类号： H01M8/16 ,  C12N11/14 ,  H01M4/8605 ,  H01M2004/8689 ,  Y02E60/527

摘要： Provided is a fuel cell having a structure in which a cathode and an anode face each other with a proton conductor therebetween. In this fuel cell, an oxygen reductase or the like is immobilized on at least the cathode, and the cathode is composed of a material having pores therein such as porous carbon. In this fuel cell, the volume of water contained in the cathode is controlled to be 70% or less of the volume of the pores of the cathode, whereby a high current value can be stably obtained through optimization of the amount of moisture contained in the cathode when an enzyme is immobilized on at least the cathode. Also provided is a method for operating the fuel cell.

摘要翻译： 提供一种燃料电池，其具有阴极和阳极彼此面对的质子导体的结构。 在该燃料电池中，氧还原酶等固定在至少阴极上，阴极由其中具有孔的材料如多孔碳构成。 在该燃料电池中，将阴极中含有的水的体积控制为阴极的孔的体积的70％以下，由此通过优化包含在阴极中的水分量，能够稳定地获得高电流值 当酶至少固定在阴极上时是阴极。 还提供了一种操作燃料电池的方法。