公开(公告)号：US10428300B2

公开(公告)日：2019-10-01

申请号：US15027485

申请日：2013-10-15

Applicant: HITACHI, LTD.

Inventor： Masaharu Kiyama ,  Guangbin Zhou ,  Takayuki Nozaki

IPC: C12M3/00 ,  C12M1/00 ,  C12M1/34

Abstract: Maintaining pressure inside a culturing vessel is difficult when minimizing the size of a liquid supply path that supplies liquid and gas to a cell culturing vessel that performs two-level culturing (co-culturing). This cell culturing device includes: culturing vessels; a flow path having at least first supply ports capable of supplying liquid or gas to the culturing vessels and first discharge ports that discharge the gas from the culturing vessels, said flow path discharging gas from the first discharge ports to the atmosphere; and a filter in the flow path. A trap bottle is provided between the first discharge ports and the filter upon the flow path to collect moisture from the discharged gas. Thus, a liquid phase and a gas phase are separated in the trap bottle; moisture is prevented from reaching the filter; filter clogging is prevented; and the internal pressure of the culturing vessels can be maintained.

公开(公告)号：US20150218503A1

公开(公告)日：2015-08-06

申请号：US14426457

申请日：2012-09-26

Applicant: HITACHI, LTD.

Inventor： Masaharu Kiyama ,  Guangbin Zhou ,  Ryota Nakajima ,  Takayuki Nozaki ,  Naoko Senda ,  Shizu Matsuoka

IPC: C12M3/00 ,  C12M1/00

CPC classification number: C12M23/42 ,  C12M23/10 ,  C12M23/22 ,  C12M23/34 ,  C12M23/38 ,  C12M23/40 ,  C12M25/04 ,  C12M29/00 ,  C12M35/08 ,  C12M37/00 ,  C12M41/48

Abstract: Provided is a cell culturing vessel for holding and culturing cells. It has a first vessel that stores a culture medium and cells or only a culture medium, a second vessel that is placed above the first vessel and stores a culture medium and cells or only a culture medium, a main vessel that holds the first vessel and houses the second vessel, and a lid member that engages with the main vessel. The main vessel has a pressing member that fixes and holds the first vessel in the main vessel and the second vessel is eccentrically held in the first vessel by the pressing member.

Abstract translation: 提供了用于保持和培养细胞的细胞培养容器。 它具有储存培养基和细胞或仅培养基的第一容器，放置在第一容器上方并存储培养基和细胞或仅培养基的第二容器，保存第一容器的主容器和 容纳第二容器，以及与主容器接合的盖构件。 主容器具有将第一容器固定并保持在主容器中的按压构件，并且第二容器通过按压构件偏心地保持在第一容器中。