公开(公告)号：US20140113281A1

公开(公告)日：2014-04-24

申请号：US14048843

申请日：2013-10-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Earl Hubbell ,  Christian KOLLER ,  Nils HOMER

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q2535/122 ,  C12Q2525/179 ,  C12Q2535/119 ,  C12Q2537/165

Abstract: A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.

Abstract translation: 用于核酸模板测序的方法包括：（a）进行第一测序过程，包括根据核苷酸和/或试剂的第一预定顺序将核苷酸和/或试剂流向核酸模板以获得第一测序结果; （b）在第一测序过程之后，进行第二测序过程，包括根据核苷酸和/或试剂的第二预定顺序将核苷酸和/或试剂流向核酸模板以获得第二测序结果，第二预定顺序 核苷酸和/或试剂不同于核苷酸和/或试剂的第一预定顺序，并且设计用于重复测序的核苷酸和/或试剂的第一和第二预定顺序中的至少一个; 和（c）使用第一测序结果和第二测序结果两者来确定对应于核酸模板的至少一部分的碱基序列。