公开(公告)号：US20140236496A1

公开(公告)日：2014-08-21

申请号：US14347864

申请日：2012-09-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gordon A. Janaway ,  Manjula Aliminati ,  Ruoyun Wu ,  David Fortescue ,  Ming Shen

IPC: G06F19/24

CPC classification number: G16B40/00 ,  C12Q1/6851 ,  C12Q2537/165 ,  C12Q2563/159

Abstract: A computer-implemented method of generating a digital polymerase chain reaction (dPCR) result is provided. The method includes detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period. The method further includes determining a positive or negative amplification determination for each sample of the plurality of samples based in part on the first set of emission data. A dPCR result is generated based on the positive or negative amplification determinations for the plurality of samples.

Abstract translation: 提供了产生数字聚合酶链反应（dPCR）结果的计算机实现的方法。 该方法包括在放大期间的第一时间，从多个样本中检测出第一组发射数据，每个采样包括多个采样区域的样本区域。 该方法还包括部分地基于第一组发射数据确定多个样本中的每个样本的正或负放大确定。 基于多个样本的正或负放大确定产生dPCR结果。