公开(公告)号：US20090162830A1

公开(公告)日：2009-06-25

申请号：US12265588

申请日：2008-11-05

申请人： Bing LIU ,  Wenjuan XU ,  Xiangping MENG ,  Yanmei QIAO ,  Mulong LIU

发明人： Bing LIU ,  Wenjuan XU ,  Xiangping MENG ,  Yanmei QIAO ,  Mulong LIU

IPC分类号： A01N1/02

CPC分类号： G01N33/5094 ,  G01N33/52 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125

摘要： A reagent for classification of leukocytes includes (a) at least two cationic surfactants; (b) at least one organic compound bearing a hydrophobic group and an anionic group; (c) a buffer for adjusting pH into a range of approximately 2-8. Also disclosed is a method for classifying leukocytes with the reagent. With the reagent and method, erythrocytes are lysed rapidly and classification of leukocytes into five groups is achieved in the same channel. The reaction may be carried out at approximately between 10-40° C. and scattered light signals may be detected at two angles for measuring the classification of leukocytes into five groups.

摘要翻译： 用于白细胞分类的试剂包括（a）至少两种阳离子表面活性剂; （b）至少一种带有疏水基团和阴离子基团的有机化合物; （c）用于将pH调节到约2-8范围内的缓冲液。 还公开了一种用于将白细胞与该试剂进行分类的方法。 利用试剂和方法，红细胞迅速裂解，白细胞在同一通道中分为5组。 反应可以在大约10-40℃进行，并且可以以两个角度检测散射光信号，以测量白细胞分为五组。